Hepatocellular carcinoma proteomic analysis

Steel, L., Shumpert, D., Trotter, M., Seeholzer, S., Evans, A., London, W., Dwek, R. and Block, T., A strategy for the comparative analysis of serum proteomes for the discovery of biomarkers for hepatocellular carcinoma. Proteomics, 3, 601-609 (2003). 

Le Naour F, Brichory F, Misek DE, Brechot C, Hanash SM, Beretta L. (2002) A distinct repertoire of autoantibodies in hepatocellular carcinoma identified by proteomic analysis. Mol Cell Proteomics 1, 197-203. 

In the present study, we analyzed proteome in hepatocellular carcinoma (HCC), esophageal cancer, and pancreatic cancer tissues. We identified many proteins whose expression in cancer tissues was different from corresponding non-cancerous tissues by using 2-DE and MS. Furthermore, we identified some auto-antibodies reacting to proteins in HCC cancer tissues. In this chapter, we will describe the method, our experimental result, and reports from other researchers about proteomic analysis in cancer patients. 

Lim SO, Park SJ, Kim W et al. Proteome analysis of hepatocellular carcinoma. Biochem Biophys Res Commun 2002 291 1031-1037. 

Li C, Hong Y, Tan YX et al. Accurate qualitative and quantitative proteomic analysis of clinical hepatocellular carcinoma using laser capture microdissection coupled with isotope-coded affinity tag and two-dimensional liquid chromatography mass spectrometry. Mo/ Cell Proteomics 2004,3399-409. 

Park KS, Kim H, Kim NG et al. Proteomic analysis and molecular characterization of tissue ferritin light chain in hepatocellular carcinoma. Hepatology 2002 35 1459-1466. 

Liang CR, Leow CK, Neo JC, Tan GS, Lo SL, Lim JW, et al. Proteome analysis of human hepatocellular carcinoma tissues by two-dimensional difference gel electrophoresis and mass spectrometry. Proteomics 2005 5(8) 2258-2271. 

Takashima M, Kuramitsu Y, Yokoyama Y, Iizuka N, Fujimoto M, Nishisaka T, et al. Overexpression of alpha enolase in hepatitis C virus-related hepatocellular carcinoma Association with tumor progression as determined by proteomic analysis. Proteomics 2005 5(6) 1686-1692. 

From proteomic analysis to clinical significance Overexpression of cytokeratin 19 correlates with hepatocellular carcinoma metastasis. Molecular Cf Cellular Proteomics MCP, 3, 73—81. 

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