Helisoma trivolvis

Goldberg, J.I., Mills, L.R. and Kater, S.B. (1991) Novel effects of serotonin on neurite outgrowth in neurons cultured from embryos of Helisoma trivolvis. J. Neurobiol. 22 182-194. 

Isol. from various molluscs incl. Helisoma trivolvis and Lymnaea stagnalis. Cardioregulatory neuropeptide. Hydrochloride 1 2) [115993-58-3]. 

Ponder, E.L., Fried, B., and Sherma, J., 2004. Thin-layer chromatographic analysis of hydrophilic vitamins in standards and from Helisoma trivolvis snails. Acta Chromatographica. 14 70-81. 

Steiner et al. (1998) used HPTLC to analyze amino acids in water conditioned by several medically important snails—Biomphalaria glabrata, Helisoma trivolvis, and Lymnaea elodes. Snail-conditioned water (SCW) provides information value (in the form of pheromones) to attract larval trematode parasites. The SCW samples were dried with air and reconstituted in 10% -propanol and then applied to cellulose HPTLC plates and developed with n-propanol-water (7 3). Amino acids were detected with ninhydrin reagent and the resulting color sample zones were compared to known standards. The amino acids present in SCW (h/ p values and color reactions with ninhydrin given in parentheses) were as follows an unknown (7, purple), aspartic acid (21, purple), serine (29, purple), alanine (40, purple), tryptophan (51, purple), valine (58, purple/orange), phenylalanine (64, light blue), and leucine (69, purple). The above amino acids were detected in the SCW of all the snails, except that phenylalanine was not detected in Lymnaea elodes. 

Conaway et al. (1995) extended the above-mentioned study of Perez et al. (1994) to determine by densitometric-HPTLC sugars in Helisoma trivolvis snails infected with the larval trematode Echinostoma trivolvis. Zarzycki et al. (1995) examined the retention properties of alpha-, beta-, and gamma-cyclodextrins using water-wettable RP-C-18 layers and mobile phases composed of acetonitrile, methanol, ethanol, or propanol with water or methanol in acetonitrile. Esaiassen et al. (1995) analyzed n-acetylchitooligosaccharides by use of the latroscan TLC/ flame ionization detection (FID) system. Brandolini et al. (1995) used automated multiple development (AMD)-HPTLC to monitor various carbohydrates—i.e., maltotetraose, maltotriose, maltose, glucose, and fructose—in different beers. 

Umesh, A., Fried, B., and J. Sherma (1996). Analysis of sugars in the hemolymph and digestive gland-gonad complex (DGG) of Biomphalaria glabrata and Helisoma trivolvis (Colorado and Pennsylvania strains) maintained on restricted diets. Veliger 39 354-361. 

Sherma et al. (1992) used TLC to identify and quantify lutein and P-caro-tene from acetone extracts of snail bodies in two strains of Helisoma trivolvis (Colorado and Pennsylvania) snails and in the Biomphalaria glabrata snail. TLC of the snail extracts on Cjg (Whatman) reversed-phase layers developed in petroleum ether-acetonitrile-methanol (2 4 4) showed a fast moving zone Ry = 0.08) identical to a P-carotene standard. Scanning of in situ spectra confirmed pigment identities. The ratio of P-carotene to lutein was 1.1 in both strains of Helisoma trivolvis, but about 4.5 in Biomphalaria glabrata. The p-carotene to lutein ratio may be useful in the chemotaxonomy of planorbid snails. 

---