Heat-Assisted Immunolabeling of Resin-Embedded Sections

MICROWAVE HEAT-ASSISTED IMMUNOLABELING OF RESIN-EMBEDDED SECTIONS 

Conventional, high-resolution immunoelectron microscopy has been extensively used for the subcellular distribution of proteins to obtain information on their functions. However, this approach is time consuming. Processing time can be substantially reduced by applying microwave heating the total time is reduced to 4-5 hr while the conventional 

FIGURE 7.6. Transmission electron micrographs of kidney tissue. (A) Conventionally stained section. (B) Section stained in a microwave oven for 30sec at 600W. Similar staining is oberved in A and B. Reprinted, with permission, from Cavusoglu et al. (1998). Copyright 1998 Blackwell Science Ltd. 

FIGURE 7.7. Thin sections of glutaraidehyde- and osmium tetroxide-fixed human biopsy heart tissue. 

TABLE 7.1. Comparative Durations of Tissue Processing by Different Protocols 

---