Hazelnut allergen

Bohle, B., Radakovics, A., Luttkopf, D., Jahn-Schmid, B., Vieths, S., and Ebner, C. 2005. Characterization of the T cell response to the major hazelnut allergen, Cor a 1.04 Evidence for a relevant T cell epitope not cross-reactive with homologous pollen allergens. Clin Exp Allergy 35(10) 1392-1399. 

Hansen, K.S., Ballmer-Weber, B.K., Ltittkopf, D. et al. 2003. Roasted hazelnuts-allergenic activity evaluated by double-blind, placebo-controlled food challenge. Allergy 58 132-138. 

Schocker, F., Liittkopf, D., Muller, U., Thomas, P., Vieths, S., and Becker, W.M. 2000. IgE binding to unique hazelnut allergens Identification of non pollen-related and heat-stable hazelnut allergens ehciting severe allergic reactions. EurJ Nutr 39 172-180. 

Vieths, S., Reindl, J., Mueller, U., Hoffmann, A., and Haustein, D. 1999. Digestibility of peanut and hazelnut allergens investigated by a simple in vitro procedure. Eur Food Res Technol 209 379-388. 

Depending on the food matrix, multiplex DNA can show some discrepancies in the detection of different allergenic targets, due to differential efficiency of the amplification process. This issue has been shown in the case of the determination of the hazelnut allergen isoforms Cor a 1.03 and Cor a 1.04 in some matrices, including dark chocolate, soy milk, lecithin supplement, and snack muesli (Bettazzi et al., 2008). For this reason, multiplex assays must be evaluated carefully during and after development, to reduce inconsistent results. 

Bettazzi F, Lucarelli F, Palchetti I, Berti F, Marrazza G, Mascini M (2008). Disposable electrochemical DNA-array for PCR amplified detection of hazelnut allergens in foodstuffs. Anal. Chim. Acta, 614(1) 93-102. 

---