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Guanine, liquid chromatography

Chen Y., Wang C., and Wu K., 2005. Analysis of N7-(benzo[a]pyrene-6-yl)guanine in urine using two-step solid-phase extraction and isotope dilution with liquid chromatography/tandem mass spectrometry. Rapid Commun Mass Spectrom 19 893. [Pg.294]

A number of lines of evidence suggested early on that 8-oxo-7,8-dihydrogua-nine (8G) is more readily oxidized than guanine [42, 86-88]. The most direct evidence in this regard was the ability to detect 8G selectively using electrochemical detectors in liquid chromatography [89]. We therefore suspected that 8G would react with metal complexes with potentials lower than... [Pg.176]

Sodum, R.S., Fiala, E.S. (2001). Analysis of peroxynitrite reactions with guanine, xanthine, and adenine nucleosides by high-pressure liquid chromatography with electrochemical detection C8-nitration and oxidation. Chem. Res. Toxicol. 14 438-50. [Pg.650]

BPDE benzo[a]pyrene diol epoxide B[a]P = benzo[a]pyrene DMSO = dimethyl sulfoxide ELISA = enzyme linked immunosorbent assay FI = fluorescence Gua = guanine GC/MS = gas chromatography/mass spectrometry HPLC = high-performance liquid chromatography NADP = oxidized nicotinamide adenosine dinucleotide ... [Pg.292]

Figure 2.5 Solid Phase DNA Synthesis Cycle (Contd.). Most frequently used base protecting groups are shown Bz Af-6 benzoyl (adenine), W-4 benzoyl (cytosine) N-2 isobutyroyl (guanine). All are base sensitive. DNA chain is built up from 3 to 5 on controlled-pore glass (CPG) bead solid support. Post global deprotection and resin removal, the desired product oligo-/polydeoxynucleotide is then separated initially by precipitation by means of an agent such as ethanol and purified finally by reversed phase liquid chromatography, or ion exchange chromatography as appropriate (see later in Chapter 2). Figure 2.5 Solid Phase DNA Synthesis Cycle (Contd.). Most frequently used base protecting groups are shown Bz Af-6 benzoyl (adenine), W-4 benzoyl (cytosine) N-2 isobutyroyl (guanine). All are base sensitive. DNA chain is built up from 3 to 5 on controlled-pore glass (CPG) bead solid support. Post global deprotection and resin removal, the desired product oligo-/polydeoxynucleotide is then separated initially by precipitation by means of an agent such as ethanol and purified finally by reversed phase liquid chromatography, or ion exchange chromatography as appropriate (see later in Chapter 2).
Folley, L.S., Power, S.D. and Poyton, R.O. (1983) Separation of nucleotides by ion-pair, reversed-phase high-performance liquid chromatography use of Mg(ll) and triethylamine as competing hetaerons in the separation of adenine and guanine derivatives, jouma/ of Chromatography 28i, 1 99-207. [Pg.73]

Harrington CF, Le Pla RC, Jones GDD, Thomas AL, Farmer PB. Determination of cisplatin 1,2-intrastrand guanine-guanine DNA adducts in human leukocytes by high-performance liquid chromatography coupled to inductively coupled plasma mass spectrometry. Chem Res Toxicol 2010 33 1313—21. [Pg.649]

Malayappan, B. et (2010) Quantitative high-performance liquid chromatography-electrospray ionization tandem mass spectrometry analysis of bis-N7-guanine DNA-DNA cross-links in white blood cells of cancer patients receiving cyclophosphamide therapy. Anal. Chem., 82 (9), 3650-3658. [Pg.223]


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