Guanidinium thiocyanate

RNA from Animal Tissues and Cells Using Guanidinium Thiocyanate... 

Extract total RNA from kidneys isolated from NTS and NSS treated mice using the guanidinium thiocyanate/acid phenol procedure (8). 

Chomczynski, P. and Sacchi, N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162, 156-159. 

Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162(1) 156—159 Liu CH, Ma WL, Shi R, Zhang B, Ou YQ, Zheng WL (2003) Gene expression study of saccaromyces cerevisiae with the Agilent 2100 Bioanalyser. Br J Biomed Sci 60(l) 22-25... 

Any RNA preparation method can be used that provides intact, undegraded RNA. Biopsy samples can be flash frozen, pulverized, and suspended in guanidinium thiocyanate prior to extraction by the Chomczynski method (24). Small, fresh samples may also be suspended in guanidinium thiocyanate. In order to shear the sample for improved extraction, the samples should be passed several times through a 22-g needle, attached to a plastic syringe. The extraction steps can be repeated several times in order to get a clean RNA preparation prior to precipitation. 

Isolation of RNA from cells or tissues is usually carried out in the presence of the chaotropic agent guanidinium thiocyanate with the purpose of disrupting the cellular structures with concomitant inhibition of ribonucleases. In this section, two basic procedures that use guanidinium thiocyanate are presented. The first is a modified version of the original procedure of Chirgwin et al.1 that is appro-... 

Transfer the sample to a 50 ml polypropylene tube and add 7.5 ml guanidinium thiocyanate lysis solution. 

Single-step guanidinium thiocyanate acid-phenol method Materials... 

Fig. 18 NOE between the guanidinium (NH2) protons and the aromatic protons a of lib as revealed by the NOESY spectrum of the 1 1 mixture of lib and guanidinium thiocyanate (500 MHz, 2 mM in acetone-, 283 K, mixing time 0.4 s)...

AI3-18430 EINECS 209-812-1 Guanidine, monothiocyanate Guanidine thiocyanate Guanidinium thiocyanate Isothiocyanic acid, compd. with guanidine (T.1) NSC 2119 Thiocyanic acid, compd. with guanidine (1 1) USAF EK-705. Potent protein denaturant used in isolation of intact DNA, RNA. Crystals mp= 117°. Dajac Labs. Eastman Chem. Co. Ftuka U.S. BioChem. 

Also, Reinhoudt and co-workers reported the data on guanidinium (thiocyanate) transport with various crowns through bulk [43] and supported [44] liquid membranes. They developed corresponding mathematical models and found that not only the host-guest complexation constant but also the lipophilicity of the host per se control ionophoric properties. Omitting mathematics, the natural reason is the possibility of carrier leakage from the membrane phase (see related work on partition coefficients and their increments for crown ethers, K-octanol/water [127]). This interplay leads, for example, to better ionophoric properties of more lipophilic... 

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