Single step guanidinium thiocyanate acid-phenol method

Single-step guanidinium thiocyanate acid-phenol method Materials 

Homogenise 100 mg tissue or 107 cells3 in 1 ml denaturing solution and transfer to a 5-ml polypropylene tube. 

Avoid the interphase and transfer the aqueous phasec to a 2 ml microfuge tube. 

Redissolve the RNA pellet in 300 pi denaturing solution and transfer to a new 1.5 ml microfuge tube. 

Redissolve the RNA pellet in 200 pi 0.25 M sodium acetate, pH 6.0 and ethanol-precipitate.d 

---

Chomczynski, P. and Sacchi, N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162, 156-159. 

Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162(1) 156—159 Liu CH, Ma WL, Shi R, Zhang B, Ou YQ, Zheng WL (2003) Gene expression study of saccaromyces cerevisiae with the Agilent 2100 Bioanalyser. Br J Biomed Sci 60(l) 22-25... 

---