Golgi method

Haberly L. 1983. Structure of the piriform cortex of the opossum. I. Description of neuron types with Golgi methods. J Comp Neurol 213 163-187. 

Fig. 1. Microphotography of a portion of the apical shaft of a pyramidal neuron of the layer V of the cerebral cortex of a rat 30 days old, showing dendritic spines distributed along it. Rapid Golgi method. 

The Golgi Atlas presents a series of camera lucida drawings of the entire telencephalon and upper brain stem of the young postnatal mouse in 24 transverse, 11 sagittal and 15 horizontal planes. The drawings were prepared from selected brains stained in toto with the Golgi method, that have been serially sectioned in the three orthogonal planes. 

The text includes an introduction of the material and methods used for the construction of this Atlas and a survey with a complete bibliography on the previous studies made with the Golgi method in Rodents. In this account, a number of issues concerning particular anatomical details are considered in relation to the interpretations obtained by other students. Reference is made to some relevant reviews and key articles. 

Most organelle membranes, such as the tonoplast (6) and the Golgi apparatus (7), can be separated by density gradient ultracentrifugation of plant cell homogenates. However, other effective methods for the isolation of the plasma membrane (8,9) have been described. Moreover, another method that uses an aqueous two-phase system for the isolation of ER is also described (10). Those interested in these details for these methods should consult the original articles. 

The study first attempted to elucidate whether the membranes of the Golgi cisternae of tobacco cells could be differentially stained by the ZIO method, and whether they were more clearly visible than the sec-... 

Fig. 2. A section of a fresh protoplast stained for 16 h by the ZIO method. A line profile shows that membranes of the Golgi cistemae (G) andERare differentially stained. (From ref. 40.)...

Akert K, Sandri C. Significance of the Maillet method for cytochemical studies of synapses, in Golgi Centennial Symposium. Proceeding (Santini M, ed.), Raven Press, New York, 1975, pp. 387-399. 

In brown algae, phlorotannins are localized in specialized bodies called physodes (Ragan 1976). Shifting the experimental approach, from chemical assays of total phlorotannin concentration to microscopic methods that describe physode transport and establish the timeframes at which phlorotannins accumulate in response to abiotic or biotic stimuli, has provided new insight into the understanding of phlorotannin production and function. It is known that physodes are derived from the endoplasmic reticulum (ER) and Golgi of the cell (Schoenwaelder and Clayton 2000). It appears that physodes are transferred across the cytosol and incorporated into the cell wall, where the phlorotannins are assumed to have a structural role and thus be involved in primary metabolism (Schoenwaelder and Clayton 2000 Arnold and Targett 2003). 

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