Glycosidases hyaluronidase

Fig. 2.8. Scaffold formation/disruption assays based on supramolecular self-assemblies. In this scheme, a cyanine is helicogenic. Molecular self-assembly of cyanine upon linear chiral polymer such as CMA, CMC, or HA results in a conformation transition of the polymer to adopt a super-helix structure. Scaffold disrupting glycosidases (hyaluronidase, amylase, cellulase) trigger fluorescence attenuation by disruption of the helical scaffold...

Thus, there appears to be an interlocking directorate with dynamic reciprocity for the various components of HA deposition that responds to the immediate needs of the cell. A tightly associated complex, termed the hyaluronasome, that contains HA receptors, the HA synthases, hyaluronidases, their respective inhibitors, exo- -glycosidases, as well as a spectrum of HA-binding proteins may constitute such an organelle. 

Another class of polymers where hydrolases are used successfully comprises polysaccharides. This approach has been pioneered by Kobayashi et al (3c,36) and is exemplified by their article (18) where hyaluronan and chondroitin were prepared via hyaluronidase-catalyzed polymerizations. A slightly different approach is to use glycosidases to prepare oligosaccharides (17,37). 

Hydrolases Glycosidase (cellulase, amylase, chitinase, hyaluronidase), lipase, peptidase, protease Polysaccharides, polyesters, polyamides, polycarbonates, poly( amino acid)s... 

The chondroitin sulphate in rat liver has been shown to be degraded by the combined actions of endo- and ej o-glycosidases, whereas that in human fibroblasts - in which hyaluronidase activity is not detected - is degraded by cxo-glycanases. 

---