Glycoprotein ovomucoid

Proteins, amino acids bonded through peptide linkages to form macromolecular biopolymers, used as chiral stationary phases for hplc include bovine and human serum albumin, OL-acid glycoprotein, ovomucoid, avidin, and cellobiohydrolase. The bovine serum albumin column is marketed under the name Resolvosil and can be obtained from Phenomenex. The human serum albumin column can be obtained from Alltech Associates, Advanced Separation Technologies, Inc., and J. T. Baker. The a1-acid glycoprotein and cellobiohydrolase can be obtained from Advanced Separation Technologies, Inc. or J. T. Baker, Inc. 

In these copolymers, the saccharide block is a carbohydrate fraction (0Q or Op) of a glycoprotein (ovomucoid) and the peptide block is a poly(benzyl-L-glutamate) block or a poly(cinnamyl-L-glutamate) block. 

Bovine serum albumin Human serum albumin a i-Acid glycoprotein Ovomucoid Avidin... 

It is well known in biochemistry that many proteins, especially enzymes but also transport proteins such as albumin, show high enantioselectivities in their interactions with small chiral molecules. It is possible to bind proteins to silica and to obtain a valuable class of CSPs that is mainly suited for the separation of chiral drugs. Several protein phases are commercially available albumins, a-acid glycoprotein, ovomucoid, avidin, cellobiohydrolase I, and pepsin. They differ in their chromatographic and enantioselective properties which is not a surprise because their biological functions and their size, shape or isoelectric point are not identical at all. 

Immobilised protein columns. A further group of chiral stationary phases has been developed by immobilising naturally occurring asymmetrical peptides, e.g. bovine serum albumin, ai-acid glycoproteins, ovomucoid and... 

Bayard investigated a number of glycoproteins (aracid glycoprotein, fetuin, lactotransferrin, transferrin, and ovomucoid) by N-deacetylation followed by deamination.176 They all gave the oligosaccharides 108 and 109, demonstrating the presence of common structural elements in these compounds. 

Balmer et al. [60] separated the two enantiomers of omeprazole on three different stationary phases with immobilized protein, viz, Chiral-AGP with a-1 acid glycoprotein, Ultron ES-OVM with ovomucoid, and BSA-DSC with BSA cross-linked into 3-aminopropyl silica using N-suc-cinimidyl carbonate. The mobile phase (1 ml/min) was phosphate buffer solution with 3—10% 2-propanol as the organic modifier. The enantiomers of omeprazole were separated on Chiralpak AD, an amylose-based chiral stationary phase, with ethanol-hexane (1 4) as mobile phase (1 ml/min). 

A number of proteins are commercially available as CSPs including a-acid glycoproteins (AGP, the major plasma binding protein for basic drugs), human serum albumin (HSA, the major plasma binding protein for weakly acidic drugs), bovine serum albumin (BSA), ovomucoid (OVM), and cellobiohydro-lase (CBH) [12]. The proteins are bonded to silica and utilized in reversed-phase mode with an aqueous buffer/organic modifier eluent. Mobile-phase... 

These proteins are composed of simple proteins in combination with carbohydrates like mucopolysaccharides, which include hyaluronic acid and chondroitin sulphates. On hydrolysis, mucopolysaccharides yield more than 4% of amino-sugars, hexosamine and uronic acid e.g., ovomucoid from egg white. Soluble mucoproteins are neither readily denatured by heat nor easily precipitated by common protein precipitants like trichloroacetic acid or picric acid. The term glycoproteins is restricted to those proteins that contain small amounts of carbohydrate usually less than 4% hexosamine. 

Since ovomucoid is a glycoprotein the solutes that can be stereo-chemically resolved on the OVM CSP are similar to the basic, neutral, and acidic compounds separated on the AGP CSE The major differences between the two CSPs appears to be quantitative i.e the magnitude of the chiral separation), rather than qualitative. The two CSPs have been recently compared and a representative series of successfully resolved solutes is contained in this article (102). 

Saliva4 Serum fraction VIC Ovomucoid" Antifreeze glycoprotein" ... 

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