Glycoproteins detection

Figure 52-3. Diagrammatic representation of the major proteins of the membrane of the human red blood cell separated by SDS-PAGE. The bands detected by staining with Coomassie blue are shown in the two left-hand channels, and the glycoproteins detected by staining with periodic acid-Schiff (PAS) reagent are shown in the right-hand channel. (Reproduced, with permission, from Beck WS, Tepper Ri Hemolytic anemias iii membrane disorders, in Hematology, 5th ed. Beck WS [editor]. The MiT Press, 1991.)...

Larvae deposit Tyv-bearing glycoproteins in cells they invade (ManWarren et al, 1997) (Fig. 6.1). Most of the glycoproteins detected in infected monolayers are ES products (Butcher et al., 2000) Tyv-bearing surface glycoproteins do not seem to be shed into cells during migration (McVay et al, 1997). Despite this evidence of contact between host cells and ES... 

In glycoprotein detection systems the carbohydrate portions of proteins are oxidized with sodium metaperiodate to generate aldehydes that can react with hydrazides. A biotin hydrazide is used to attach biotin onto the oxidized carbohydrates and horseradish peroxidase-conjugated streptavidin is used for chemiluminescence-based detection (Glycoprotein Detection Module, Amersham Biosciences, Uppsala, Sweden). 

Spik, G., M. Monsigny, and J. Montreuil Investigation of glycoprotein. Detection of a linkage between aspartic acid and the mucopolyosidic groups in human transferrin. Comp. Rend. Soc. Biol. 260, 4282 (1965). 

Glycoprotein detection using diagnostic oxonium ions... 

Clegg, J. C. S. (1982) Glycoprotein detection in nitrocellulose transfers of electrophoretically separated protein mixtures using concanavalin A and peroxidase Application to arenavirus and flavivirus proteins. Anal. Biochent. 127, 389-394. 

Luker GD, Fracasso PM, Dobkin J, Piwnica-Worms D. Modulation of the multidrug resistance P-glycoprotein detection with technetium-99m-sestamihi in vivo. JNucl Med 1997 38 369-372. 

LC-MS with on-line SPE using a RAM pre-column with an internal ODS phase was described by van der Hoeven et al. (95) for the analysis of cortisol and prednisolone in plasma, and arachidonic acid in urine. The samples were injected directly and the only off-line pretreatment required was centrifugation. By using the on-line SPE-LC-MS system, cortisol and related compounds could be totally recovered and quantified in 100 p.1 plasma within 5 min with a typical detection of 2 ng/ml (Figure 11.6(b)). The RAM-type of sorbents, in which the outer surface of the particles is covered with aj-acid glycoprotein, also appear to be useful for direct SPE of... 

Periodic acid-Schiff reagent Detects glycoproteins as pink bands after electrophoretic separation. 

Incubation of cultured cells with glycoproteins as radioactive bands Leads to detection of a radioactive sugar after electrophoretic separation. 

Lampio, A. and Finne, J., Sugar analysis of glycoproteins and glycolipids after methanolysis by high-performance liquid chromatography with pulsed amperometric detection, Anal. Biochem., 197, 132, 1991. 

Pathogen-suppressing oligosaccharides in human milk were quantitated using capillary Electrophoresis by MEKC with detection by absorption at 205 nm.90 A 24 kDa glycoprotein associated with muscle-wasting cachexia... 

A linkage involving 4-hydroxy-L-proline and L-arabinose has been detected in glycoproteins of plant origin, and it is also possible that L-cys-teine is part of a carbohydrate-protein linkage. Of the types of linkages just mentioned, which have been extensively discussed in reviews,2-9 only the first three have been the subject of synthetic studies, and it is this aspect of the work that will be discussed in the present Chapter. 

Wu, Z., Nagano, I. and Takahashi, Y. (1998) The detection of Trichinella with polymerase chain reaction (PCR) primers constructed using sequences of random amplified polymorphic DNA (RAPD) or sequences of complementary DNA encoding excretory-secretory (E-S) glycoproteins. Parasitology 117, 173-183. 

---