Glycolipid analysis

Figure 13. Protein and enzyme curves for epithelial cells fractionated from small intestine of the two rat strains. Experimental conditions were modified from Weiser (4). Pooled fractions used for glycolipid analysis are defined at the top. Data were from single rats.

Glycolipid analysis. Gangliosides were extracted with chloroform-methanol (2 1), purified on Sephadex G-25 columns and separated into individual species by thin-layer chromatography as described previously (12). 

The majority of the performed TLC/MALDl studies of glycolipids used UV lasers. However, there are also a few studies where IR lasers were applied. Beside an IR laser, Dreisewerd and coworkers [48] used an orthogonal but not an axial M ALDI MS for glycolipid analysis. It was found that even minor gangliosides from a complex lipid mixture (extracted from cultured Chinese hamster ovary cells) could be characterized directly from the TLC surface. 

Tingey, a. Results of glycolipid analysis in certain types of lipidosis and leukodystrophy. J. Neurochem. 3, 230 (1959)... 

Lampio, A. and Finne, J., Sugar analysis of glycoproteins and glycolipids after methanolysis by high-performance liquid chromatography with pulsed amperometric detection, Anal. Biochem., 197, 132, 1991. 

Wang, W. Liu, Z. Ma, L. Hao, C. Liu, S. Voinov, V. G. Kalinosvskaya, N. I. Electrospray ionization multiple-stage tandem mass spectrometric analysis of digycosyldiacylglycerol glycolipids from the bacteria Bacillus pumilus. Rapid Comm. Mass Spectrom. 1999,13,1189-1196. 

METHYLATION TECHNIQUES IN THE STRUCTURAL ANALYSIS OF GLYCOPROTEINS AND GLYCOLIPIDS... 

Methylation analysis (and its different applications) is one of the most widely used methods in the structural characterization of carbohydrate chains (reviewed in refs, 5-8). In the present article, the progress achieved, and the problems encountered, in the analysis of animal glycolipids and glycoproteins are discussed, although many of the topics also concern the analysis of carbohydrate chains in general. Emphasis is laid on the efforts made to find ways to lessen the amounts of sample needed for the analysis, because only small amounts of glycan samples isolated from biological sources are often available. 

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