Glycan release

A. M. Taylor, O. Holst, and J. Thomas-Oates, Mass spectrometric profiling of O-linked glycans released directly from glycoproteins in gels using in-gel reductive p-elimination, Proteomics, 6 (2006) 2936-2946. 

The separation of ANTS-derivatized glycans released from bovine RNase B, bovine fetuin, and chicken ovalbumin was reported by means of ion-pair RPLC with trimethylammonium as ion-pairing agent [79]. 

N., Leon, V, and Pujol, J.P. An inositol-phosphate glycan released by TGF-beta mimics the proliferative but not the transcriptional effects of the factor and requires functional receptors. Cell Signal,... 

The analysis of the highly complex mixture of glycans released from tissues by extensive proteolytic degradation was for a long time complicated by the lack of... 

Profiling of expressed glycans released from glycoproteins includes the analysis of glycan composition and sequence, the linkages between monosaccharides, and the distribution of glycoforms. 

MS provides measurement of the molecular weight for glycans. The saccharide composition for glycans released from glycoproteins (or glycolipids) can be determined based on the accurate mass, the structures of known N- or O-linked glycans, and the core structure, as well as the masses of the monosaccharides. In addition, tandem MS can confirm the proposed composition and provide further details of the structure. 

Figure 4 Positive ion MALDI mass spectrum of AAlInked glycans released from ribonuclease B and recorded from (A) 2,5-DHB and (B) esculetin.

Generally, for more detailed glycoprotein MS analyses, two main approaches exist (i) glycan release followed by characterization of the whole pool and (ii)... 

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