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Glycans release from glycopeptide

Nakano, M. Kakehi, K. Lee, Y. C. Sample clean-up method for analysis of complex N-glycans released from glycopeptides. J. Chromatogr. A 2003, 1005, 13-21. [Pg.758]

The glycans were released from glycopeptides by gas phase hydrazinolysis (100 C, 2 hr) with Hydraclub C-206 (Honen Co., Tol o, Japan), followed by N-acetylation. The pyridylamino (PA) derivatives of these glycans with 2-aminopyridine were prepared with a PALSTATION model 4000 (Takara Co. Kyoto, Japan) (17). The PA-glycans were purified by HPLC on a normal phase column. [Pg.411]

Fig. 32. General mechanism for the alkaline P-elimination used for the release of a glycan from an O-linked glycopeptide. Fig. 32. General mechanism for the alkaline P-elimination used for the release of a glycan from an O-linked glycopeptide.
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Glycan release

Glycane

Glycans

Glycans release

Glycopeptide

Glycopeptides

Released glycans

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