Glutaraldehyde GA

To avoid, or diminish the fouling process, a blend of PVA with cellulose (CELL) has been obtained by spin coated of the PVA (M=50 000 99% hydrolysis degree) / glutaraldehyde (GA) solution (corresponding to 0.005 or 0.01 moles of GA/ mole of PVA... 

Figure 2. Comparison of DSC thermograms for native and glutaraldehyde (GA)-crosslinked / -D-glucosidase at pH 5.0 in lOmM NaCl. In order to facilitate comparison of the peaks, the differential heat capacity values plotted for the native enzyme are the actual values multiplied by a fector of 0.571.

Neeper-Bradley TL, Butler BL, Fisher LG, et ah Two-generation reproduction study with glutaraldehyde (GA) in the drinking water of CDN rats. Toxicologist 15(1) 165, 1995... 

Troupe and his coworkers reported a two-step method to electrochemically deposit GOx enzyme on a diamond film electrode (Troupe et al. 1998). In the first step, the GOx and BSA were dissolved in a buffer with pH = 7, greater than their isoelectric points. The BDD electrode was immersed into the solution and biased at 3.5 V for 2 h. Therefore, the negatively charged proteins (GOx and BSA) were drawn to the positively charged electrode. In the second step, the glutaraldehyde (GA) cross-links the protein layer forming a water insoluble barrier. 

In many cases, silicon organic compounds are used as connecting links between a silica surface and a biomolecule. The most popular technique utilizes the modification of a hydroxy-functionalized surface with 3-aminopropyltriethoxysilane (APTES) [2] followed by crosslinking with glutaraldehyde (GA) (Fig. 1 A). 

A Immobilization with 3-aminopropyltriethoxysilane (APTES) and glutaraldehyd (GA) B Immobilization using aldehyd-fimctionalized ethoxysilanes... 

The homobifunctional cross-linker, glutaraldehyde (GA), can be used for conjugating the molecules containing primary amine groups with amine-terminated PMMA. This is where the formation of Schiff bases with possible rearrangement to a stable product or through a Michael-type addition reaction occurred. The reaction takes place at points of double-bind unsaturation created by polymerization of the GA in solution (121-122) (reaction shown in scheme 8.4). 

It is often assumed that the two-step glutaraldehyde (GA) method is superior to the NaI04 method to obtain 1 1 conjugates of antibody-... 

Many applications rely on enzymes being retained by membranes, aggregated by cross-linking, or immobilized by encapsulation. These techniques are often simple and inexpensive, but typically also generate a poorly defined immobilized enzyme. The immobilization can involve isolated enzymes or whole cell preparations. Sweetzyme IT, an immobilized glucose isomerase produced by Novozymes is an example of the latter, in which the cells are cross-linked by glutaraldehyde (GA) and extruded to produce dry, solid particles [32]. 

Bioprostheses used in heart valves Processing of animal tissues to reduce biodegradability and antigenicity for the bioprostheses used in heart valves has been most successfully achieved by glutaraldehyde (GA) fixation. Because tissue length may be shortened as much... 

Cotton fabric -HTCC along with different cross-linking agents including citric acid (CA], BTCA and glutaraldehyde (GA] in the presence of a proper catalysts (NaHjPOj or MgClj]... 

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