Glutamic plasminogen

Zivin JA, Mazzarella V. Tissue plasminogen activator plus glutamate antagonist improves outcome after embolic stroke. Arch Neurol 1991 48 1235-1238. 

Figure 10.13 Separation of the tryptic hydrolysate of tissue-type plasminogen activator [reproduced with permission from R.L. Garnick, N.J. Solli and P.A. Papa, Anal. Chem., 60,2546 (1988)]. Conditions stationary phase. Nova PakClS, 5 pm mobile phase Imlrnin" 50 mM sodium phosphate pH 2.8-acetonitrile, step gradient UV detector 210 nm. Top the normal protein with arginine at position 275 below the mutant with glutamic acid at position 275.

GTC Biotherapeutics Human antithrombin, Human serum albumin, HIV vaccine, malaria vaccine, Monoclonal antibodies, Peptides, Fusion proteins. Beta-interferon, Interferon alpha. Glutamic acid decarboj lase. Human growth hormone. Insulin, Tissue plasminogen activator 9, 40-49... 

Desialylation destroys the protease activity of plasminogen. Functional prothrombin arises from its precursor by two post-translational modifications a vitamin K-dependent carboxylation of glutamic acid residues, followed by glycosylation in the carboxylated region (prothrombin fragment 1 AS 1-156). Deglycosylation of prothrombin in the presence of Ca " leads to greatly increased self-association this occurs at certain contact points on the molecule, which are probably concealed by carbohydrate chains in the intact molecule (Fletcher et al... 

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