Glutamate spectrum

Examining the mass spectrum of glutamic acid-TBDMS shows two high-mass ions that are 28 mass units apart (see Figure 9.3). By adding 57 (C4H9) Daltons to the m/z 432 ion, the MW of the... 

It was straightforward to identify the spin systems of four valines, five threonines, four alanines, three glycines, two glutamates, and AMX type residues (Asp, Cys, Phe, Tyr, Trp, and Ser) of this protein from the COSY, RELAY, and NOESY spectra in D O solution. The RELAY spectrum was particularly useful in identification of Val, Ala, Thr, and Glu spin systems. 

Zonisamide is a broad-spectrum sulfonamide AED that blocks voltage-sensitive sodium channels by reducing voltage-dependent T-type Ca channels it also weakly inhibits carbonic anhydrase, and inhibits glutamate release. 

The heparin and poly-L-glutamate titrations show a markedly different behavior than do the DNA titrations. As polyanion is added, the fluorescence of the an-thrylpolyamine solution decreases until a well-defined minimum is reached. A new emission at 510 nm, which we assign to the anthracene excimer of 14, increases and decreases coincidently with the titrated fluorescence minimum. Likewise, the UV spectrum of 10 fiM 14 with added heparin shows hypochromism that occurs and disappears coincidently with the fluorescence minimum and a 2-nm red shift. We have proposed template-directed excimer formation as the physical basis for these observations. In the absence of heparin, fluorescence of the unassociated probe is observed. As heparin is added, the fluorescence decreases as a result of heparin-directed interaction between probe molecules. Additional heparin permits the fluorophore population to diffuse over the length of the poly anion, thus avoiding excimer formation and yielding a net CHEF. 

Fig. 4. 500 MHz DOSY spectrum of a D2O solution of a perchloric acid extract of gerbil brain. Assignments of selected signals are indicated as follows ac = acetate ala = alanine cho = choline cr = creatine ere = creatinine etn = ethanolamine GABA = 7-aminobutyric acid glu = glutamate GPC = glycerophosphocholine lac = lactate m-ino = myo-inositol NAA = N-acetylaspartate succ = succinate and tau = taurine. (Raw data...

Circular dichroism studies of the [ZnL (phen)2](2 ")+ (HL = chiral amino acid) systems have been reported.251 A molecular structure determination of Cd glutamate dihydrate shows the Cd to be coordinated in a square pyramidal fashion by the two oxygens and two nitrogens of the glutamate and the water molecule.252 The IR spectrum of [Zn-(gtycineglydnate)X] H20 has been assigned.253... 

Other published EPR data show that L-glutamate produces a small axial distortion on the environment of enzyme-Mn(II) with unresolved fine structure and no obvious change in line width. Addition of MgATP results in a diminution of all spectral intensities and the anisotropic spectrum shows poorly resolved fine structure splitting. The appearance of these additional sets of transitions indicates that the environment of Mn2 + at the tight site is changed when metal nucleotide is bound. NH4 + produces additional subtle changes in the EPR spectrum of Mn2+. 

A) The absorbance spectrum of poly-y-methyl-L-glutamate in the a-helical form, with an assignment of its three constituent transitions. (B) The far-UV CD spectrum of the same compound, deconvoluted on the assumption that the shapes of the absorbance and dichroic bands are directly related (broken lines). The solid line is the observed spectrum and the filled circles the sum of the three bands. In general, the sign of a CD band may be either positive or negative, and its intensity does not necessarily follow that of the absorbance band. (From Holzwarth and Doty, 1965.)... 

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