Glucosyl transferase

FIGURE 6.20 A disaccharide of galactose and glucose is covalently linked to the 5-hydroxyl group of hydroxylysines in collagen by the combined action of the enzymes galactosyl transferase and glucosyl transferase. 

Another interesting point is that glucosyl transferase and galactosyl transferase apparently exerted different activities depending on the blueberry cultivar. ... 

E. A. MacGregor, H. M. Jespersen, and B. Svensson, A circularly permuted alpha-amylase-type alpha/beta-barrel structure in glucan-synthesizing glucosyl-transferases, FEBS Lett., 378 (1996) 263-266. 

Figure 6.1 Major branch pathways of flavonoid biosynthesis in Arabidopsis. Branch pathways, enzymes, and end products present in other plants but not Arabidopsis are shown in light gray. Abbreviations cinnamate-4-hydroxylase (C4H), chalcone isomerase (CHI), chalcone synthase (CHS), 4-coumarate CoA-ligase (4CL), dihydroflavonol 4-reductase (DFR), flavanone 3-hydroxylase (F3H), flavonoid 3 or 3 5 hydroxylase (F3 H, F3 5 H), leucoanthocyanidin dioxygenase (LDOX), leucoanthocyanidin reductase (LCR), O-methyltransferase (OMT), phenylalanine ammonia-lyase (PAL), rhamnosyl transferase (RT), and UDP flavonoid glucosyl transferase (UFGT).

Pflugmacher S, Sandermann H Jr (1998) Taxonomic distribution of plant glucosyl-transferases acting on xenobiotics. Phytochemistry 49 507-511... 

Monosaccharide attachment to some of the hydroxylysine residues within the a chains occurs at this stage as galactose (gal) and glucose (glc) residues are added by specific galactosyl transferase and glucosyl transferases. The carbohydrates are attached as single monosaccharides or as gal-glc disaccharides. 

Jourdan PS, Mansell RL (1982) Isolation and partial characterization of three glucosyl transferases involved in the biosynthesis of flavonol tiiglucosides in Pisum sativum L. Arch Biochem Biophys 213 434-443... 

Also referred to as UDP-glucose-/3-D-glucan glucosyl-transferase and UDP-glucose-cellulose glucosyltransfer-... 

The properties of bilirubin UDP-glucosyl- and bilirubin UDP-xylosyl-transferase from rat liver closely parallel each other with regard to activation by digitonin and dependence on pH and bivalent metal ions. Considerable fractions of the enzyme activities (especially of the glucosyl-transferase) functioned independently of added bivalent cation (F3). The observations are compatible with identical enzyme locations, at least for the metal ion-stimulated activities. 

Figure 1. Postulated pathway for the enzymatic synthesis of polymethylated flavonol glucosides in Chrysosplenium americanum GT, O-glucosyl-transferase OH, hydroxylase OMT, O-methyl transferase.

Mitsunaga, T. et al., Inbibitory effects of bark proanthocyanidins on the activities of glucosyl-transferases of Streptococcus sobrinus, J. Wood Chem. Technol, 17, 327, 1997. 

Nagatoshi M, Terasaka K, Nagatsu A, Mizukami H. An Iridoid-Specific Glucosyl-transferase from Gardenia jasminoides. JBC July, 2011 1-19. Available from http // www.jbc.org/cgi/doi/10.1074/jbc.Mlll.242586 (accessed 20 September 2012). 

CDG-Ic Dol-P-Glc Man9GlcNAc2-PP-dolichol glucosyl-transferase hALG6 lp22 604566... 

Figure 3-14. Possible biosynthetic route towards coumarin. (a) 2-hydroxylase, (b) glucosyl transferase, (c) P-glucosidase, (d) dimethylallyl transferase and/or UV light. The enzymes have not yet been identified.

The pathogenesis of dental caries may involve three distinct processes (1) adherence of the bacteria to the tooth, (2) formation of glycocalyx due to synthesis of a sticky glucan by the action of the bacterial enzyme glucosyl transferase on sucrose, and (3) accumulation of biobUm (plaque), within which there is continuing acid production by constituent bacteria (including streptococci and lactobacflli) able to metabolize carbohydrates at low pH values. This acid demineralizes an enamel. 

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