Glucose with hemoglobin, reaction

Glucose reacts non-enzymatically with amino acids and proteins, including hemoglobin, egg-white proteins and serum albumin. For example, if glucose is not removed prior to drying, dried egg whites slowly turn brown and develop off-flavors and odors. What do you propose as the most likely first step in the non-enzymatic glucose-protein chemical reaction How can the first product transform spontaneously into a ketose derivative ... 

Many clinical chemistry assays are based on UVAfIS spectrophotometry, often by reaction of the chemical of interest, snch as glucose, with an enzyme and dye to create a colored complex. A novel method for determining the age of dried bloodstains at a crime scene has been developed by researchers at the National Center for Forensic Science at the University of Central Horida using the Implen NanoPhotometer Pearl (Hanson and Ballantyne). The researchers discovered a previously unidentified hypsochromic shift (a blue shift to shorter wavelengths) in the Soret band of hemoglobin =412... 

Creatine phosphokinase activity has been reported to be minimally inhibited by hemolysis. Hemoglobin concentrations of 1.25 g/100 ml inhibit 5% and 2.5 g/100 ml, 12% (N5). However, in methods utilizing adenosine diphosphate in the reaction mixture, hemolysates containing 100 mg of hemoglobin per 100 ml may have apparent activities of 5-100 units/liter. The activity is presumably related to adenylate kinase in the erythrocyte (S33). In methods utilizing adenosine diphosphate in a coupled enzyme reaction with hexokinase and glucose-6-phosphatase, the inhibitory effect can be eliminated by adding sufficient adenosine mono-... 

Several different glycolytic intermediates such as glucose-6-phosphate, fructose-6-phosphate, fructose 1,6-diphosphate and glyceraldehyde-3-phosphate were proposed to serve as substrates. The direct reaction of hemoglobin with glucose is much slower and less specific than that with glucose-6-phosphate. 

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