Glucose oxidase catalysis, activity

Kohen, A., Jonsson, T. and Klinman, J.P. (1997). Effects of protein glycosylation on catalysis changes in hydrogen tunnehng and enthalpy of activation in the glucose oxidase reaction. Biochemistry 36, 2603-2611... 

The preparation and application of SAM systems patterned by STM and their use in catalysis was demonstrated by Wittstock and Schuhmann [123]. The patterning (local desorption) of SAMs from alkane thiols on gold was performed by scanning electrochemical microscopy (SECM), followed by the assembly of an amino-deriva-tized disulfide and coupling of glucose oxidase to form a catalytically active pattern of the enzyme. The enzymatic activity could be monitored/imaged by SECM. 

Roth J. P Klinman J. P. Catalysis of electron transfer during activation of 02 by the flavoprotein glucose oxidase. Proc. Nat. Acad. Sci. USA 2003, 100, 62-67. 

Immobilized CPO and glucose oxidase (GOx) on SBA-15 were mixed for tandem catalysis By the use of the tandem system, a conversion of 92% of indole was reached at pH = 5.5. Also, the mixture of immobilized CPO and GOx was recycled several times without significant loss of activity [9]... 

All electrodes react with their environment via the surfaces in ways which will determine their electrochemical performance. Properly selected surface modification can effectively enhance the electrode heterogeneous catalysis property, especially selectivity and activity. The bulk materials can be chosen to provide mechanical, chemical, electrical, and structural integrity. In this part, several surface modification methods will be introduced in terms of metal film deposition, metal ion implantation, electrochemical activation, organic surface coating, nanoparticle deposition, glucose oxidase (GOx) enzyme-modified electrode, and DNA-modified electrode. 

The glucose - glucose oxidase system is probably the best studied example of catalyst activity maintenance. Evaluation of system behavior, based upon Henri-related reactions (3.5a, 3.5b) and deactlvatlons (eqns 3.5c, 3.5d) occurring simultaneously with internal (eqns 3.5a,b) diffusion and external mass transfer presents as complete a kinetic analysis as is conventionally available in other well studied commercial examples of catalysis. 

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