Glucose lectins

Glucose oxidase, glycoproteins from plasma membranes of rat liver Analytical and semi preparative separations Lectin Affinity (Concanavalin A) disks [76]... 

Lectins Acute Increased Heat Mobilisation of phase sympathetic production 0 Fuels proteins activity (glucose +... 

Gottschalk, 1., Li, Y. M., Lundahl, P. Chromatography on cells analyses of solute interactions with the glucose transporter Glutl in human red cells adsorbed on lectin-gel beads. 

Another interesting and recent application of ACE is the fluorescence-enhanced competition assay for the detection of sugar-lectin interactions (77). Because glucose competes with the fluorescently labeled dextran for the lectin-binding sites, the relative fluorescent intensity due to the displaced dextran is proportional to the concentration of glucose. 

To immobilize a protein the biologic activity of which depends on a cofactor, this cofactor has to be present in sufficient concentration during the immobilization step. Thus, for example, the lectin Concanavalin A is immobilized in the presence of a buffer containing 0.1 M glucose or a-methylmannoside, 1 mM calcium chloride and 1 mM manganese chloride. 

Sephacryl (allyldextran cross-linked by N,N -methylenebisacrylamide) are the two types of dextran gel used in separation. Sephadex is mainly used as a glucose pol)rmer and employed for purification of many molecules such as lectins from Helix pomatia and Viciafaba, exoamylase from Pseudomonas stutzeri [14]. 

A report on the inhibitory effect of wheat-genn agglutinin on cellular transport may be relevant to this study, as this lectin binds to 2-aeet-amido-2-deoxy-D-glucose present in the same, L-asparagine-linked class of oligosaccharide that is affected by tunicamycin.549... 

In the purification of lectins, special adsorbents have been employed. The adsorbents have been prepared by attaching specific, carbohydrate ligands to activated supports.41-106,157 An affinity adsorbent prepared from chitobiose and (2-aminoethyl)-Bio-Gel was used to purify the 2-acetamido-2-deoxy-D-glucose-binding lectin from wheat germ. The data for the purification of this lectin are shown in Fig. 6. 

Fig. 6.—Elution Pattern for Wheat-germ Lectin from an Adsorbent Prepared from N,N -Diacetylchitobiose and (2-Aminoethyl)-Bio-Gel by Reductive Animation. [Initial elution with phosphate buffer in saline, followed by elution with 0.1 M 2-acetamido-2-deoxy-D-glucose in phosphate buffer and saline (arrow) (see Ref. 25).]...

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