Glucose cultivation media

Thiamine was biosynthesized by resting cells of S. typhimurium strain thilO/T-ath-383, which can synthesize thiamine from exogenous glucose, AIRs, and thiazole.54 Derepression was achieved by conventional means. The organism was cultivated in the presence of a suboptimal amount of thiamine (20 nM), the washed cells were resuspended in a minimal medium containing glucose (10 mM), thiazole (1-2 mM), and labeled AIRs (10 p,M). During the incubation (1.5 hours 37°C), the level of thiamine diphosphate in the cells had risen from about 0.04 to 0.5 nmol/mg. In work with molecules labeled with stable isotopes, thiamine was extracted and cleaved by ethanethiol to 4-amino-5-(ethyl-... 

Stress is a broad term often used with animal cells. Frequently mechanical forces are meant using this term but chemical stress is also important cultivating animal cells. The chemical environment of the cell in a reactor have to be considered very carefully. The complexity of the medium requirements and the metabolic pathway cause very often growth limitations. Studying these limitations in order to find the reasons showed to be difficulty because of the complexity of the system. Nevertheless, glucose, glutamine, lactate and ammonia are found to be critical parameter as well as the osmotic pressure. 

It is difficult to interprets the result obtained when the SCPP strain was cultived in the presence of 0.1% glucose because its growth on such a medium was severely reduced. 

Culture conditions The exo-1 strain was cultivated in two-stages (I) pre-cultivation for 24 hours in Vogel s medium [7] supplemented with 2% glucose, and (II) transfer of the mycelial... 

There is considerable interest in synthesizing copolymers. This is actually possible if organisms are confronted with mixtures of so-called related and unrelated substrates. Copolymers can also be synthesized from unrelated substrates, e.g., from glucose and gluconate. The 3-hydroxydecanoate involved in the polyester is formed by diversion of intermediates from de novo fatty-acid synthesis [41,42]. Related , in this context, refers to substrates for which the monomer in the polymer is always of equal carbon chain length to that of the substrate offered. Starting from related substrates, the synthesis pathway is closely connected to the fatty-acid /1-oxidation cycle [43]. In Pseudomonas oleovor-ans, for example, cultivated on octane, octanol, or octanoic acid, the synthesized medium chain length polyester consists of a major fraction of 3-hydroxyoc-tanoic acid and a minor fraction of 3-hydroxyhexanoic acid. If P. oleovorans is cultivated on nonane, nonanol, or nonanoic acid, the accumulated polyester comprises mainly of 3-hydroxynonanoate [44]. 

Although alternative expression systems have been successfully adapted for the production of isotope-labeled proteins (see Sect. 1.5), heterologous expression in E. coli often remains the method of choice for NMR sample preparation. There is a fundamental difference, however, with respect to the kind of medium in which the cells are cultivated. In a so-called chemically defined or minimal medium only one or a very limited number of carbon sources is provided, e. g. glucose or glycerol. All bacterial metabolites have to be biosynthesized by the cells through the various, sometimes lengthy and energy-de-... 

We optimized the cultivation protocol with respect to aqueous medium composition, organic phase, and phase ratio (the ratio of the volume of the organic phase to the total liquid volume). The best system consists of a defined mineral medium, with glucose as the carbon source and diethylhexylphthalate as the organic phase at a phase ratio of 0.5. The organic phase contained 2 vol % styrene and 1 vol % of octane, which we added as an inducer for gene expression. 

Pseudomonas spp. DSM 6611 and 6978 and Rhodococcus ruber DSM 44541 were cultivated in shaking flasks for 3 days at 30 °C with shaking at 120 rpm in YPG medium containing 10 g yeast extract, 10 g bacteriological peptone, 10 g glucose. 

Production of Lignin Peroxidase. Medium for the inoculum was rich in yeast extract (25 g/1) and glucose (25 g/l) to promote maximal growth of the mycelia. The inoculum of Phanerochaete chrysosporium ATCC 24725 was first cultivated for 3 days at 30°C in five litres of medium divided in five shake flasks. The shake flask batches were transferred to a 100 litre bioreactor and cultivated again for 3 days at 30°C. The batches were stirred and aerated to obtain maximal growth of mycelia. 

You are going to cultivate yeast, Saccharomyces cerevisiae, by using a 10 m -fermenter your company already owns. You want to find out the amount of ethanol the fermenter can produce. Therefore, a chemostat study was carried out and the Monod kinetic parameters for the microorganism grown in the glucose medium at 30°C, pH 4.8, were found to be Ks = 0.0025 g/L and /imax = 0.25 h-1. The ethanol yield (YP/S) is 0.44 (g/g) and cell yield (Yx/S) is 0.019 (g/g). The inlet substrate concentration is 50 g/L-... 

FPU/mL) vs time for aerobic batch cultivation of T. reesei Rut-C30. The initial growth medium was a Mandels medium with 10 g/L of glucose as the carbon source. At t = 67 h, Solka-floc was added to a concentration of 10 g/L. 

---