Glucose chromatography

Ligand exchange Equihbrium Chromatographic separation of glucose-fructose mixtures with Ca-form resins Removal of hea y metals with chelating resins Affinity chromatography... 

Maltose (H2O) [6363-53-7] M 360.3, m 118 . Purified by chromatography from aqueous soln on to a charcoal/Celite (1 1) column, washed with water to remove glucose and other monosaccharides, then eluted with aqueous 75% EtOH. Crystd from water, aqueous EtOH or EtOH containing 1% nitric acid. Dried as the monohydrate at room temperature under vacuum over H2SO4 or P2O5. 

Glucose oxidase (from Aspergillus niger) [9001-37-0] Mf 186,000, [EC 1.1.3.4]. Purified by dialysis against deionized water at 6° for 48hours, and by molecular exclusion chromatography with Sephadex G-25 at room temperature. [Holt and Cotton J Am Chem Soc 109 1841 1987.]... 

There are two types of stationary phases commonly used in exclusion chromatography silica gel and micro-reticulated cross-linked polystyrene gels. A third type of exclusion media is comprised of the Dextran gels. Dextran gels are produced by the action of certain bacteria on a sucrose substrate. They consist of framework of glucose units that can form a gel in aqueous solvents that have size exclusion properties. Unfortunately the gels are mechanically weak and thus, cannot tolerate the high pressures necessary for HPLC and, as a consequence, are of very limited use to the analyst. 

Defaye, K. and Garcia Fernandez, J.M., Protonic and thermal activation of sucrose an the oligosaccaride composition of caramel. Carbohydrate Res., 256, Cl, 1994. Ratsimba, V. et al.. Qualitative and qnantitative evaluation of mono- and disaccharides in D-fructose, D-glucose and sucrose caramels by gas-liquid chromatography-mass spectrometry di-D-fructose dianhydrides as tracers of caramel authenticity, J. Chro-matogr. A, 844, 283, 1999. 

MAE in conjunction with high-performance liquid chromatography/mass spectrometry (HPLC/MS) shortened the cleanup procedure and provided satisfactory recoveries (97-102%) for the parent imazethapyr and its two metabolites (hydroxy and glucose conjugate metabolites). 

D. Fabbri, G. Chiavari, S. Prati, I. Vassura and M. Vangelista, Gas chromatography/mass spectrometric characterisation of pyrolysis/silylation products of glucose and cellulose, Rapid Commun. Mass Spectrom., 16, 2349 2355 (2002). 

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