Gluconic dehydrogenase

W17. Willmer, J. S., Changes in hepatic enzyme levels after adrenalectomy. II. Glucose-6-phosphatase, glucose-6-phosphate dehydrogenase, and 6-phospho-gluconate dehydrogenase. Can. J. Biochem. and Physiol. 38, 1449-1456 (1960). 

This enzyme [EC 1.1.1.126], also known as 2-keto-3-deoxy-D-gluconate dehydrogenase, catalyzes the reaction of 2-dehydro-3-deoxy-D-gluconate with NADP to produce (45,55 )-4,5-dihydroxy-2,6-dioxohexanoate and NADPH. 

Fig. 9. Sequence of transformations catalysed by the supramolecular ATP-generating system [38, AcP, Mg2, ADP] (38 = [24]-N6C>2) and the enzymes hexokinase (HK), glucose-6-phosphate dehydrogenase (G-6-PDH) and 6-phospho-gluconate dehydrogenase (6-P-GDH) [5.32],...

The electrocatalytic oxidation of D-gluconic acid at an ubiquinine-mixed carbon paste electrode with an immobilized layer of D-gluconate dehydrogenase has been described.259... 

T. Ikeda, K. Miki, F. Fushimi, and M. Senda, Electrocatalytic oxidation of D-gluconate at a ubiquinone-mixed carbon paste electrode with an immobilized layer of D-gluconate dehydrogenase from bacterial membranes, Agric. Biol. Chem., 51 (1987) 747-754. 

Figure 6. 51V NMR spectra of 0.5, 1.0, and 5.0 mM total vanadate in 150 mM imidazole at pH 8.0. The V2 (—555 ppm) and V4 (—579 ppm) resonances are indicated by arrows. The V2 resonance (—573 ppm) is upfield of the Vj and downfield of the V4 resonance, and the V5 resonance (—585 ppm) is furthest upfield. The H+-dependent equilibrium constants K12 and K14 are defined, and plots of [V2] as a function of [ V2]2 and [V4] as a function of [V2]4 are shown for a study carried out at constant ionic strength. The data shown in these plots were reported previously as assay conditions for studies of 6-phospho gluconate dehydrogenase from sheep liver (Data are from reference 35).

Mediatorless amperometric measurement of gluconate, alcohol and fructose was demonstrated in the simple configuration of a carbon paste electrode when gluconate dehydrogenase, alcohol dehydrogenase and fructose dehydrogenase, respectively, were immobilized behind a membrane [6]. 

Ikeda, T.. Eushimi, F., Mild, K., and Senda, M. (1988) Direct bioelectrocatalysis at electrodes modified with D-gluconate dehydrogenase. Agricultural and Biological Chemistry, 52 (10), 2655-2658. 

Ikeda, T., Miyaoka, S., and Mild, K. (1993) Enzyme-catalyzed electrochemical oxidation of D-gluconate at electrodes coated with D-gluconate dehydrogenase, a membrande-bound flavohemoprotein. Journal of Electroanalytical Chemistry, 352 (1-2), 267-278. 

G, and Naresh Kumar, G. (2013) 2-Ketogluconic acid secretion by incorporation of Pseudomonas putida KT 2440 gluconate dehydrogenase (gad) operon in Enterobacter asburiae PSI3 improves mineral phosphate solubilization. Curr. Microbiol, 67 (3), 388-394. 

Fig, 17.6 Hexose monophosphate pathway. 1, glucose-6-P-dehydrogenase 2, 6-P-gluconolactonase 3,6-P-gluconate dehydrogenase 4, phosphoribose isomerase 5, phosphoketopentose epimerase 6, transketolase 7, transaldolase TPP, thiamine pyrophosphate. 

Therefore, an innovative two-step fermentation process were discovered to resolve this problem. In the new innovative two-step fermentation process, the D-glucose was converted into 2,5-diketo-gluonic acid (2,5-DKG) by Erwinia herhicola ATCC 21988 or some similar strains with glucose dehydrogenase, gluconate dehydrogenase... 

---