Glucagon immunoreactive insulin

Since in vitro studies with perfused rat pancreas (Fig. 1) have shown that immunoreactive insulin (IRI) is released rather instantaneously when glucose or glucagon are added to the perfusate (Grodsky and Bennett, 1966), an attempt was made to investigate in man the very early phase of insulin release during the first 5 minutes after stimulation (Simpson et al., 1967). We characterized the early insulin response to intravenous glucose in normal human subjects, in subjects with diabetic heritage, lean noninsulin dependent diabetics (Simpson et al., 1966), and nondiabetic obese patients (Bendetti et al, 1967). 

Fig.. 2, Effect of glucagon on serum immunoreactive insulin (IRI), free fatty acid (FFA) and glucose in normal dogs.

This test revealed that the olive oil group had better glycaemic tolerance than the coconut oil in both oral and duodenal glucose tolerance tests. It was also demonstrated that an increased secretion of gut glucagons-like immunoreactivity (gGLI) was needed to value the GLP-1 levels, in the olive oil rats compared to coconut oil rats. Nevertheless, issue levels of GLP-1, plasma insulin and glucagon were the same between two rats treated groups. 

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