Gliadin detection

TABLE 19.1 Commercially Available Test Kits for Gliadin Detection"... 

Laube, T., Kergaravat, S.V., Fabiano, S.N. et al. (2011) Magneto immunosensor for gliadin detection in gluten-free foodstuff towards food safety for celiac patients. Biosens. Bioelectron., 27, 46-52. 

MG Scanlon, W Bushuk. Application of photodiode-array detection in RP-HPLC of gliadins for automated wheat variety identification. J Cereal Sci 12 229-234, 1990. 

Henterich et al. [51] returned to the initial AbiPrism 7700/TaqMan system for the detection of gliadin in food samples, with a 30-fold gain in sensitivity... 

Osman, A.A., Uhlig, H., Thamrn, B., Schneider-Mergener, J., Mothes, T. 1998. Use of the phage display technique for detection of epitopes recognized by polyclonal rabbit gliadin antibodies. FEBS Lett 433 103-107. 

Kahlenberg F, Sanchez D, Lachmann I, Tuckova L, Tlaskalova H, Mendez E, et al. Monoclonal antibody R5 for detection of putatively coeliac-toxic gliadin peptides. Eur Food Res Technol 2006 222 78-82. 

The fingerprinting of gliadins and glutenins in various wheat flours by LC-MS shows complex traces with more than 20 peaks. The two major components, i.e., and Ys gliadin, were identified through A-terminal sequencing and accurate-mass determination [52]. The components were proposed as markers to detect traces of wheat in gluten-free food preparations. 

Although Osborne s fractionation was a major milestone in the development of cereal chemistry, it must be understood that each of these fractions is a complex mixture of different polypeptides, and also that these polypeptides overlap in their solubilities. This is particularly true for the gliadins and glutenin proteins. Beckwith et al. (1966) detected by gel filtration studies a small fraction of the ethanol-soluble proteins as an excluded peak, which he called gliadin of high molecular weight (HMW) [5]. Nielsen et al. (1968) found that... 

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