Glass surface, immobilization

Fig. 17 (a) Elastin-based stimulus-responsive gold nanoparticles. Reproduced from [131] by permission of The Royal Society of Chemistry (b) Functionalization of a glass surface with ELP. In the first step, the glass surface is aminosilylated with N-2-(aminoethyl)-3-aminopropyl-trimethoxysilane, then modified with glutaraldehyde. Subsequently, the stimulus-responsive biopolymer is covalently immobilized using reductive amination. Reproduced from [132] by permission of The Royal Society of Chemistry... 

In another investigation, ELP[V5L2G3-90] with three lysines in the N-terminal region was immobihzed on a glass surface in a microreactor to enable temperature-controlled positioning of ELP fusion proteins. For this purpose, the glass surface was first functionalized with A -2-(aminoethyl)-3-aminopropyltrimethoxysilane, followed by glutaraldehyde treatment and reductive amination to immobilize the biopolymer on the surface (Fig. 17b) [132]. 

Amorphous Systems (Polymer and Surface Immobilized Catalysts and Inorganic Glasses)... 

Immobilization on glass surfaces can be carried out by either routes [5] or [6] in Scheme 2 both yielding the immobilized complex (1), reaction [5] being the procedure most commonly used. 

An array or a matrix of nucleic acid probes immobilized at discrete locations on a silicon or glass surface provides a convenient means to simultaneously probe a sample for the presence of many different target sequences. Microarray biochip scanning devices, mostly based on fluorescent labels, are now currently available, and could also be used with CL labels to take advantage of the higher sensitivity of this detection principle. 

A sensor array where different haptens are immobilized at well-defined areas on a plain glass surface has been developed [66], Using an automated flow injection system it was possible to incubate all areas on the chip with analytes, specific antibodies, secondary HRP-labeled antibodies, and CL substrate. Measurement of the light output via imaging performed with a CCD device allowed determination of the analytes present in the sample on the basis of the spatial localization of the CL signal. 

A. A. Tsygankov, Y. Hirata, Y. Asada, J. Miyake (1993) Immobilization of the purple nonsulfur bacterium Rhodobacter sphaeroides on glass surfaces. Biotechnol. Techn., 7 283-286... 

Fig. 14.5 Surface chemistry The OFRR glass surface is biofunctionalized with 3APS. Then biorecognition molecules are immobilized using a cross linker...

Measurement of the kinetic rates and surface diffusion of reversibly bound biomolecules at flattened biological and model membrane surfaces and at specifically derivatized glass surfaces (e.g., with immobilized enzymes). 

Fig. 10 a Scheme of dendrimer generation 4 and b description of surface activation to fix the dendrimers and immobilization method for covalent binding of NAs on the activated glass surface [42]... 

Fig. 21 a Fluorescence images of xCP-immobilized fluorescein labeled ONDs on a glass surface. The pattern size is limited only by the use of manufactured molds with the desired feature size, b AFM images of stamped 1 p.m lines of 20-bp ONDs (left) and 500bp PCR fragments (right) on mica substrates [79]. Reprinted with permission... 

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