Stained glass

All glassware, including coplin jars, glass-staining racks, and stacks of cover glass and bottles for buffers, should be heat treated in an oven at 180°C for 8 h. The items can be covered by aluminium foil before being placed in the oven in order to prevent contamination when removing the items afterward. 

Tissue sections (8 pan thick) are placed on Probe-on Plus slides and floated on autoclaved water on a hot plate (42°C) for 2-3 min to remove compression. The water is removed with a paper towel, and the slides are placed on a slotted glass staining dish on its side in the microwave oven. To adhere the sections, the slides are heated in a microwave... 

Using the glass staining racks and tanks place slides into the lysis solution for approximately 15 min (see Note 7). 

Cutting of glass wire glass, stained glass, laminated glass, etc... 

Industries of luxury goods, such as artistic glass, leaded glass, stained glass, jewelry glass, crystal glass, and so forth. 

Distressing Stains. Interest and charm can be added to furniture by the dehberate infliction of imperfections. These imperfections may be caused by physical distress such as hammers, files, nails, chains, or rocks or caused by finish distress such as "dy specking" or staining to simulate past abuse, such as waterstains caused by the careless placement of a drinking glass. 

Water Tests. In colorfastness to water, ISO 10S-E01, the test specimen is placed in contact with the chosen adjacent fabrics, immersed in water, and placed wet between glass plates and left for 4 h at 37°C. After drying, the effect on the test specimen and stain on adjacents are assessed. The test, colorfastness to seawater, ISO 10S-E02, is the same as EOl but uses 30 g/L anhydrous sodium chloride solution instead of water. To test for colorfastness to chlorinated seawater/swimming baths water, ISO 10S-E03, the specimen is immersed in sodium hypochlorite solution containing either 100, 50, or 20 mg of active chlorine per Hter at pH 7.5 for 1 h at 27°C, rinsed, dried, and assessed. 

Protein residues, eg, soft-boiled egg yolk, are difficult stains to handle. If the stains are not totally denatured, proteases can decompose them. There are commercial proteases with a high temperature optimum (60°C) that can remove most protein soils in a dishwasher (63). Patents on the use of Upases in ADDs have claimed that Upases can reduce the formation of spots and films on glasses (62,64—66) however, no commercial appUcation of Upases in ADDs has been implemented. 

Artifacts introduced through sample preparation are common materials these may be bits of facial tissue, wax, epithelial cells, hair, or dried stain, all inadvertently introduced by the microscopist. Detergent residues on so-called precleaned microscope slides and broken glass are common artifacts, as are knife marks and chatter marks from sectioning with a faulty blade, or scratch marks from grinding and polishing. 

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