Giemsa staining

Figure 36-12. Sister chromatid exchanges between human chromosomes. These are detectabie by Giemsa staining of the chromosomes of ceiis repiicated for two cycies in the presence of bromodeoxyuridine. The arrows indicate some regions of exchange. (Courtesy of S Wolff and J Bodycote.)...

Fig. 5 BALB/c 3T3 cell transformation assay example of focus induced in BALB/c 3T3 clone A31-1-1 after exposure to a carcinogenic compound. Cells are stained with Giemsa stain...

Thick and thin films are best stained with Giemsa stain, as it provides the most detailed and intense staining of parasites. Wright stain can be used for thin films but not for thick films, as it contains alcohol, which will fix the erythrocytes. Wright stain does not stain parasites as well as Giemsa stain. The staining procedure is outlined below. 

Aspirates of bone marrow or spleen may be useful in the diagnosis of infections such as leishmaniasis, trypanosomiasis, and occasionally malaria. In such instances, Giemsa stains of alcohol-fixed bone marrow films are most useful. Splenic aspiration is rajrely performed in the United States because it is dangerous. 

Prepare a 1 40 dilution of stock Giemsa stain in neutral buffered water, pH 7.0 to 7.2 (generally, 2 ml of Giemsa stock plus 38 ml of buffered water with 0.01% Triton X-100). 

Stain the film for approximately 60 min (the time, which will vary slightly with different lots of stock Giemsa stain, can be determined by the staining of leukocytes and erythrocytes). 

Prepare a 1 50 dilution of stock Giemsa stain in neutral buffered water (pH 7.0 to 7.2). 

Blood Malaria and babesiasis Send unstained and, if available, Giemsa-stained thick and thin films. Fix thin film... 

Much of the confusion was caused by the inadequacy of the staining procedures. By means of the HCl-Giemsa staining technique of Piekarski, many observations have been reported demonstrating the presence of chromatinic structures in bacteria. 

Giemsa stain chem A stain for hemopoietic tissue and hemoprotozoa consisting of a stock glycerol methanol solution of eosinates of Azure B and methylene blue with some excess of the basic dyes. gem-s3, stan gifblaar poison See fluoroacetic acid. gif,blar. poiz-on ... 

In order to evaluate the frequency and quality of chromosome spreads, stain one of the air-dried slides following the instructions that accompany the Diff Quik stain set (total time about 1 min) or stain with Giemsa stain (3). 

Rowley JD. Tetter A new consistent chromosomal abnormality in chronic myelogenous leukaemia identified by quinacrine fluorescence and Giemsa staining. Nature 1973 243 290-293. 

Figure 7. M813 infection induces fusogenicity in vitro (A) PA317 cells incubated with M813 for 4 h. (B) Uninfected PA317 cells. Giemsa staining. Magnification 150. Reprinted from (Prassolov et al., 2001b) with permission from Elsevier.

Giemsa Stain - [PHOTOGRAPHY] (Vol 18) -for white blood cells [AUTOMATED INSTRUMENTATION - HEMATOLOGY] (Vol 3)... 

Horobin, R. W., and Boon, M. E. 1988. Understanding microwave-stimulated Romanowsky-Giemsa staining of plastic embedded bone marrow. Histochem. J. 20 329-334. 

Figure 4. Photomicrograph of 3T3 murine fibroblasts stained with Wright s-Giemsa stain a) treated with W. floribunda agglutinin (150 ng/ml) in Eagle s Minimum Essential Medium with Hanks salts for 48 hr b) control...

Prepare Giemsa stain fresh 6.6 mL up to 500 mL with buffer. 

May-Grunwald-Giemsa staining will show all of the infiltrating cells in the lung. 

Figure 1-3. Partial karyotypes of Giemsa-stained human chromosomes showing various manifestations of the fragile X site (arrows) a chromatid break (a), an isochromatid gap (b), a chromosome break (c), and endoreduplication (d).

Approximately 24,48, or 72 hours after dosing, mice killed and bone marrow obtained from both femurs Slide of a bone marrow suspension prepared, stained with Wright-Giemsa stain, dried, and scored blind... 

Direct fluorescent antibody smears have become a more efficient method than Giemsa stains or tissue cultures fiar identifying chlamydia. Commercially prepared kits make specimen collection convenient, and results are available in approximately 24 hours. Good results, however, depend on obtaining an adequate specimen. Fluorescein-labeled monoclonal antibodies in the staining reagent specific for Chlamydia trachomatis outer membrane proteins bind to the C. trachomatis in the smear. Studies that compare direct fluorescein antibody techniques with tissue culture results have found acceptable sensitivity and specificity values. 

Ocular Smear Interpretation for Gram and Giemsa Stains... 

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