GFP labeling

Enenkel C, Lehmann A, Kloetzel PM (1999) GFP-labelling of 26S proteasomes in living yeast insight into proteasomal functions at the nuclear envelope/rough ER. Mol Biol Rep 26 131-135... 

Variations on the filter-based assay have been designed to approximate more physiological contexts. Such assays include tumor cell invasion across a confluent cell monolayer (e.g., endothelial cells (EC) as a surrogate for intravasation or extravasation during hematogenous metastasis (24)) and ovarian carcinoma invasion of mesothelial cell monolayers (25). Additionally, 1 mm thick slices of human brain tissue have been used as a tissue barrier on Transwell filters with invasion of GFP-labeled glioma cells measured by confocal microscopy (26). 

Colonization of gfp labeled E. coli 0157 H7 at cut edges of leafy greens No surface sterilization Confocal microscopy to detect gfp label Takeuchi and Frank (2000)... 

Greene LE, Park YN, Masison DC, Eisenberg E (2009) Application of GFP-labeling to study prions in yeast. Protein Pept Lett 16 635-641... 

Prepare GFP-labeled cells as mentioned previously with the... 

Peters, V. and Rehm, B.H.A. (2005) In vivo monitoring of PHA granule formation using GFP-labeled PHA synthases. FEMS Microbiol Lett., 248, 93-100. 

Fig. 9.7 Phyllosoma infected by Vibrio owensii, tagged with green-fluorescent protein (GFP) to visualise progress of infection. Healthy phyllosoma, early infection (left), with few GFP-labelled ceils and late infection (right), with mass proliferation and infection of hepatopancreas tubules (light grey area within hepatopancreas).

Once the behaviour of the fluorescent label was understood the authors moved to study GFP labelled PH within a living cell—in this case a mouse myoblast. Figure 6.5(a) shows the individual spots of light that were observed at the plasma... 

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