Genome phenotype

Forward chemical genomics Phenotype or response-driven Traditional drug discovery Molecular target unknown or poorly characterized Phenotypic assays, organ bath, tissue binding assays, etc. Target for Probe ... 

Begley TJ, Rosenbach AS, Ideker T, Samson LD. Damage recovery pathways in Saccharomyces cerevisiae revealed by genomic phenotyping and interactome mapping. Mol Cancer Res 2002 1(2) 103-12. 

Compositional patterns were called genome phenotypes (Bernard and Bernard , 1986a), because they are different in different vertebrate classes (see also Part 4). It should be stressed that, until the work of Thiery et al. (1976), the common belief was that, while DNA composition varied greatly among bacteria, animal species had a rather similar DNA composition (Kit, 1960). Although some small variations in modal buoyant densities and standard deviation of CsCl profiles had been detected in vertebrate DNAs, these could be due to different methylation levels (Kit, 1962). 

As already mentioned in Chapter 1, the eompositional changes, the major shifts, that took place between cold- and warm-blooded vertebrates, and the maintenance of the compositional patterns so formed cannot be accounted for by any explanation only or essentially relying on stochastic processes. This led us (Bernard and Bernard , 1986a) to propose a natural selection mechanism acting on the genome phenotype. 

Keywords. MetabolicEngineering, Functional, Genomics, Phenotype, Systems, Biology... 

A study that investigates the statistical associations between a phenotype and a very large number of genetic markers supposed to inform on the global variability of the genome. Because GWA studies do not rely on a priori knowledge, they may lead to the discovery of new causes of disease. 

Classical global knockouts may have a developmental or lethal phenotype and thus preclude the analysis of the phenotypic consequences of the lack of a gene in specific tissues in adult animals. With the development of the cre/loxP and flp/FRT systems, it has become possible to excise defined DNA fragments from the genome of specified cells. Cre and Flp are bacterial and yeast recombinases, respectively, which recognize loxP and FRT sequences, respectively. The most common... 

Famili I, Forster J, Nielsen J, Palsson BO. Saccharomyces cerevisiae phenotypes can be predicted by using constraint-based analysis of a genome-scale reconstructed metabolic network. Proc Natl Acad Sci USA 2003 100 13134-9. 

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