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Genome analysis sequence interpretation

Interpretation of results of these studies is still difficult. Results of two-hybrid methods become more useful if they can be coordinated with other approaches. For example, computational methods can predict interactions from genome sequences alone. 11/0 More than 45,000 interactions have been predicted among yeast proteins. Reliable identification of such motifs as DNA-binding domains and Ca2+- binding domains can complement two-hybrid analysis.11 The yeast genome is predicted to contain 162 coiled-coil sequences and at least 213 unique interactions between them.0 Examination of sequences of protein families in the Protein Data Bank (PDB) led to prediction of 8151 interactions of 664 types between protein families in yeast.P... [Pg.1726]

The current situation in bioinformatics is characterized by an avalanche of DNA sequences from the human genome project and similar programs and, consequently, an exponential increase in DNA sequences but only a linear increase in protein 3D structures. While multitudes of putative genes have been annotated, up to 90% of all known DNA sequences have no assigned, i.e., experimentally proven, function. From this situation arise the need for interpretation of DNA sequences by information technology, and moreover, analysis of functional genomics and proteomics (see Chapter 15). [Pg.417]

Comparative genomics, the analysis of DNA sequence patterns of humans and other model organisms, has become one of the most powerful strategies for identifying human genes and interpreting their function. [Pg.285]


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