Genome analysis metabolic pathway

Toward Genome-Scale Metabolic Pathway Analysis 111... 

In summary, the system among many other things, enables to (i) assign primary functions based on cross-genome analysis, (ii) assert metabolic pathways and function... 

C. H. Schilling, S. Schuster, B. O. Palsson, and R. Heinrich, Metabolic pathway analysis Basic concepts and scientific applications in the post genomic era. Biotechnol. Prog. 15, 296 303 (1999). 

The property of cold acclimation resulting in freezing tolerance has evolved in many temperate plants. The metabolic pathways of this complex process are mostly unknown. However, from recent work it is clear that gene activation is involved. By means of molecular biology some cold-regulated transcripts have been isolated and have become amenable to further analysis. The cDNA clones so far available probably correspond to only a small number of the transcripts which are affected by low temperature. One may therefore expect that the number of cDNA clones and genomic clones available for characterisation will increase in the near future. 

Most recently, in silico methods have been apphed to the systems biology of metabolic pathways. An analysis of the human genome assigns 2709 human enzymes to 896 bioreactions 622 of the enzymes are assigned roles in 135 predicted metabolic pathways. The predicted pathways closely match the known nutritional requirements of humans. This analysis identifies probable omissions in the human genome annotation in the form of 203 pathway holes (missing enzymes within the predicted pathways). The predicted human metabolic map is described by a Pathway/Genome Database called HumanCyc, which is available at http //HumanCyc.org/. 

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