Genetics and regulation of enzyme

A prerequisite for a successful genetic modification of the producing strains is a thorough understanding of the distribution and regulation of the metabolic fluxes and the identification of limiting steps (Fig. 2). Ahrens et al. [56] and Menzel et al. [57] studied the in vivo and in vitro activities of the key enzymes... 

Biochemical and genetic investigations the structure, function, and regulation of specific extracellular enzymes. 

The chemical reactions in living cells are organized into a series of biochemical pathways. The pathways are controlled primarily by adjusting the concentrations and activities of enzymes through genetic control, covalent modification, allosteric regulation, and compartmentation. 

Hayashi, T., K. Salata, A. Kingman, and A. L. Notkins. 1988. Regulation of enzyme levels in the blood influence of environmental and genetic factors on enzyme clearance. American Journal of Pathology 132 503-511. 

Possible functions of secondary products in the metabolism of the producing organisms were discussed in a number of papers. Several different experimental approaches were developed to study a possible role of antibiotics and other secondary products in the producing cells. These include the genetic and biochemical analysis of mutants blocked in the synthesis of secondary metabolites, and the study of metabolic pathways and regulation of the synthesis and activity of enzymes involved in the production of secondary metabolites. 

The microenvironment of secondary metabolic enzymes plays an important role in the direction and regulation of secondary metabolism. Genetic experiments and in vivo investigations with isotope-labeled compounds indicate the existence... 

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