General Aspects of PHA Recovery

3 Recovery of PHA from Biomass 7.3.1 General Aspects of PHA Recovery 

Downstream processing constitutes a key part of the entire PHA production process. After biosynthesis of the polyester and separation of the bacterial biomass (normally by well-established techniques like centrifugation, sedimentation, flocculation or filtration), the process required for PHA recovery from the microbial cell mass must be considered as an important cost factor, especially considering large scale production. Choosing the adequate method for separating PHAs from residual biomass is dependent on several factors the production strain, the required product purity that is determined by the final application of the biopolymer, the 

Carbon rich waste stream Occurrence Main convertible carbon source after upstream processing Microbial production strain References 

Lignocellulosics Sugar bagasse, rice straw, fruit crops, corn straw, fruit seed production ffexoses, pentoses, sugar alcohols Pseudomonas pseudoflava, Burkholderia sacchari IPT 101 and Burkholderia cepacia IPT 048 [31 33] 

Crude glycerol phase Biodiesel production Glycerol Haloferax mediterranei. Bacillus sphaericus, Methylobacterium rhodesianum Halorcula sp., Methylobacterium extorquens, Novosphingobium capsulatum, Cupriavidus necator. Pseudomonas oleovorans. Pseudomonas corrugata [35, 42 6) 

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