Gene-locus mutation assays thymidine kinase

Myhr B, Bowers L, Caspary WJ. 1985. Assays for the induction of gene mutations at the thymidine kinase locus in L5178Y mouse lymphoma cells in culture. In Ashby J, de Serres FJ, et al., eds. Progress in mutation research. Vol. 5. Evaluation of short-term tests for carcinogens. Amsterdam, The Netherlands Elsevier Science Publishers, 555-568. 

The mouse lymphoma (MOLY) assay is an in vitro mammalian cell gene mutation test that can be used to detect gene mutations induced by chemical substances. The cell line used is the L5178Y MOLY cell. In these cell lines the most commonly used genetic end points measure mutation at the thymidine kinase (TK) locus on the mouse chromosome 11b. 

MBOCA induced gene mutations at the thymidine kinase (TK) locus in mouse lymphoma cells (Caspary et al. 1988 Myhr and Caspary 1988). Unscheduled DNA synthesis (UDS) was induced in HeLa cells (Martin and Mcdermid 1981), in rat primary hepatocytes at >10 pmol (McQueen et al. 1981 Mori et al. 1988 Williams et al. 1982), and in hamster (McQueen et al. 1981) and rabbit (McQueen and Wiliams 1987) hepatocytes. The concentration that tested positive in the mouse was 50 jmol (McQueen et al. 1981). Sensitivity to MBOCA showed species-specific variations rat > mouse > hamster > rabbit (McQueen et al. 1981, 1983). Because hepatocytes have their own metabolic activation systems, no exogenous metabolic activation is needed. In assays using attachment independence as an end point, MBOCA, at concentrations near the LC o, transformed baby hamster kidney (Daniel and Dehnel 1981 Styles 1981), rat embryo (Dunkel et al. 1981 Traul et al. 1981), and Balb/3T3 cells (Dunkel et al. 1981). Transformation assays have not been evaluated... 

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