Genes inactivation

Ribosome protection (tetM gene) inactivating tetracyclines... 

The fact that molecular components are organized in pathways, networks and complexes used for different tasks in the organism explains, at least partly, the existence of pleiotropy as shown in particular by gene inactivation studies. 

Jones, A., Korpi, E. R., McKeman, R. M., et al. (1997) Ligand-gated ion channel subunit partnerships GABAa receptor a6 subunit gene inactivation inhibits delta subunit expression. J. Neurosci. 17,1350-1362. 

Itiee, J. M., et ah. Parkin gene inactivation alters behaviour and dopamine neurotransmission in the mouse. Hum Mol Genet, 2003, 12(18), 2277-91. 

Apart from gene silencing, other effects of DNA methylation include spontaneous deamination, enhanced DNA binding of carcinogens and increased UV absorption by DNA, all of which increase the rate of mutations, DNA adduct formation and subsequent gene inactivation... 

Figure 8.3 Erythromycin derivatives obtained by gene inactivation.

Figu re 8.6 Tylosin derivatives obtained by combining gene inactivation, gene expression and feeding experiments. 

Figure 8.8 Methymycin/pikromycin derivatives generated by gene inactivation.

The effect of elevated extracellular 5-HT concentration on the modulation of programmed cell death during neural development was also investigated in early postnatal brains of 5-HTT KO mice. 5-HTT gene inactivations leads to a reduced number of apoptotic cells in striatum, thalamus, hypothalamus. 

Several families of homeodomain genes expressed in the anterior part of the CNS, the prosencephalon, have now been described (Fig. 1.2). Their function has been determined in the mouse by gene inactivation studies. 

Dietzl G, Chen D, Schnorrer F, et al. (2007) A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. Nature 448,151-6. 

Even more elegantly, site-specific recombinases may be used to structurally disrupt a gene which had previously been provided with recombinase-specific sites, for example targeted to intronic sites (see also Section 2.2.4). Deletion of flanked exons, and consequently gene inactivation, will however... 

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