Disruption of gel structure

Sonication or rough chemical treatment can be used for solubilization of conven- 

however, quantitation via gel disruption is foreseen, it is advisable to replace the Bis cross-linking reaction with another one that yields more easily solubilizable gels. Ethylenediacrylate is used for this purpose [253]. Dissolution can be done either by the action of concentrated NH4OH [254] or by piperidine-alcoholic hyamine [253] (9 1). In the latter case gels are dissolved by incubation at 37°C for 3-4 h. Dissolution can be accelerated by addition of another portion of piperidine. 

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