Gene delivery transfection

Key words Lipoplex, siRNA, Liposome, Gene delivery. Transfection... 

Keywords Gene delivery Transfection Lipid Polymer therapeutics DNA plasmid Cationic polymers Cytotoxicity. 

AV Kabanov, FC Szoka Jr, LW Seymour. In PF AV Kabanov, LW Seymour, eds. Interpoly-electrolyte Complexes for Gene Delivery Polymer Aspects of Transfection Activity. Chichester, UK Wiley, 1998, pp 197-218. 

S. S. Diebold, H. Lehrmann, M. Kursa, E. Wagner, M. Cotten, and M. Zenke, Efficient gene delivery into human dendritic cells by adenovirus polyethyleni-mine and mannose polyethylenimine transfection, Hum. Gene Ther., 10 (1999) 775-786. 

Bennett, M.J., Aberle A.M., Balasubramaniam R.P., Malone, J.G., Malone, R.W., and Nantz, M.H., Cationic lipid-mediated gene delivery to murine lung correlation of lipid hydration with in vivo transfection activity, Journal of Medicinal Chemistry, 1997, 40, 4069 1078. 

Isobe H, Nakanishi W, Tomita N, Jinno S, Okayama H, Nakamura E (2006a) Gene delivery by aminofullerenes Structural requirements for efficient transfection. Chem. Asian J. 1 167-175. 

A more selective inhibition of NFkB can be achieved by transfecting cells with DNA coding for the natural inhibitor IkBo or a mutant IkB protein that lacks 36 N-terminal amino acids, and consequently becomes proteolysis resistant. In this way expression of adhesion molecules and monocyte adhesion and transmigration can be inhibited [87,88], The potentials and limitations of these latter types of therapy are however not fully understood as yet. Different transfection systems (adenoviral, retroviral, non-viral) are available for gene delivery purposes, all with their own potentials and restrictions. 

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