Gene clusters, biosynthetic cloning

The above proposal was confirmed some years later when Floss and coworkers reported studies in which the entire asukamycin biosynthetic gene cluster was cloned, assembled, and expressed heterologously in S. lividans (Figure 3.74) [260]. DNA... 

Becker, J.E., Moore, R.E. and Moore, B.S. (2004) Cloning, sequencing, and biochemical characterization of the nostocyclopeptide biosynthetic gene cluster molecular basis for imine macrocyclization. Gene, 325, 35 42. 

Karolewiez, A., and Geisen, R. (2005). Cloning a part of the ochratoxin A biosynthetic gene cluster of Penicillium nordicum and characterization of the ochratoxin polyketide synthase gene. Syst. Appl. Microbiol. 28, 588-595. 

Luzhetskyy A, Mayer A, Hoffmann J et al (2007) Cloning and heterologous expression of the aranciamycin biosynthetic gene cluster revealed a new flexible glycosyltransferase. ChemBioChem 8 599-602... 

Erb A, Luzhetskyy A, Bechthold A et al (2009) Cloning and sequencing of the biosynthetic gene cluster for saquayamycin Z and galtamycin B and the elucidation of the assembly of their saccharide chains. ChemBioChem 10 1392-1401... 

Ogasawara Y, Katayama K, Minami A et al (2004) Cloning, sequencing, and functional analysis of the biosynthetic gene cluster of macrolactam antibiotic vicenistatin in Streptomyces halstedii. Chem Biol 11 79-86... 

Abe, Y., Suzuki, T., Ono, C., Iwamoto, K., Hosobuchi, M., and Yoshikawa, H. (2002). Molecular cloning and characterization of an ML-236B (compactin) biosynthetic gene cluster in Penicillium citrinum. Mol Genet Genomics 267 636-646. 

Widdick DA, Dodd HM, Barraille P, White J, Stein TH, Chater KF, Gasson MJ, Bibb MJ. Cloning and engineering of the cinnamycin biosynthetic gene cluster from streptomyces cinna-moneus cinnamoneus DSM 40005. Proc. Natl. Acad. Sci. U.S.A. 

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