Gelatin Spermine

Scaffolds can also be used for the delivery of genes. Kido et al. prepared scaffolds of gelatin and (3-tricalcium phosphate ((3-TCP) to search their gene transfection capabilities. The scaffolds were loaded with a spermine-pullulan luciferase plasmid DNA polyion complex and then stem cells were seeded into the scaffolds. It was shown that the level of plasmid DNA transfection was dependent on the method of the scaffold preparation. ... 

Nanostructures containing cationized gelatin are able to protect pDNA and siRNA from nuclease degradation and transfect a variety of cell types and tissues, both in vitro and in vivo. After cationization with ethylenediamine or spermine, the levels of transfection efficiency achieved can be compared to those provided by classic transfection agents such as polyethyleneimine. Table 8.2 shows the most important results obtained with nanoparticles based on cationized gelatin for gene delivery. 

Table 8.2 Compilation of studies employing gelatin cationized with the amines cholamine (CA), ethylenediamine (ED) or spermine (SPM).

Seki, T., et al., Effects of a sperminated gelatin on the nasal absorption of insulin. International Journal of Pharmaceutics, 338 213-218,2007. 

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