Gel electrophoresis of nucleic acids

Rill, RL Ramey, BA Van Winkle, DH Locke, BR, Capillary Gel Electrophoresis of Nucleic Acids in Pluronic F127 Copolymer Liquid Crystals, Chromatographia Supplement I, Vol 49, S65, 1999. 

Rickwood D, Hames BD (eds.) (1984) Gel electrophoresis of nucleic acids a practical approach. IRL Press, Oxford Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning a laboratory manual, 2nd ed., vol. 1, chap. 6, Cold Spring Harbor Laboratories Press Jany K-D, Hahn H (1991) In Bertram S, Gassen G (eds.) Gentechnische Methoden. pp. 39-43. Fischer, Stuttgart... 

Rickwood D, Homes BD (eds.) (1984) Gel electrophoresis of nucleic acids a practical approach. IRL Press, Oxford Voytas D (2000) Current protocols in molecular biology. 2.5A. WUey, New York... 

To understand how these modem methods work, it is necessary first to review some general laboratory techniques ubiquitous in genetic engineering. Among the most important are gel electrophoresis of nucleic acids, nucleic acid hybridization assays, and the polymerase chain reaction. 

Chapter 8. The practice of analytical gel electrophoresis of nucleic acids. . . 359 Chapter 9. Practice of preparative gel electrophoresis of nucleic acids. 408... 

D. Grierson, in Gel Electrophoresis of Nucleic Acids A Practical Approach, D. Rickwood and B. D. Hames, Eds., Oxford University Press, New York, 1982, pp. 29-31. 

Riesner D, Steger G, Zimmat R, Owens RA, Wagenhofer M, Hillen W, et al. Temperature-gradient gel electrophoresis of nucleic acids analysis of conformational transitions, sequence variations, and protein-nucleic, acid interactions. Electrophoresis 1989 10 377-89. 

Details of DNA electrophoresis methods are given in Table 9.3. The most widely used buffers in gel electrophoresis of nucleic acids are Tris/acetate/EDTA (TAE) and Tris/borate/EDTA (TBE). TBE has the best buffering capacity but the use of TAE tends to result in somewhat sharper bands. For some purposes, such as DNA extraction with glassmilk, TBE should be avoided unless sorbitol is... 

Gel electrophoresis of nucleic acids at this step often reveals the presence of visible bands of ribosomal RNAs as well as high molecular weight DNA. 

Gel electrophoresis of nucleic acids and phosphoproteins has been developed with great success over the past two decades (below) (Figure 14.9). This technique has been particularly helpful in the understanding of the composition of viruses. 

Gel Electrophoresis of Nucleic Acids Electrophoresis in Denaturing Media... 

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