Galactopyranoside nitrophenyl

Hydrolysis and Transglycosylation Investigated in Micro Reactors Organic synthesis 27 [OS 27] Hydrolysis of p-nitrophenyl-y D-galactopyranoside... 

The micro channel may be pre-treated before processing in the following way first, the channel is charged with /3-galactosidase in phosphate buffer then, only buffer solution is passed finally, only p-nitrophenyl-/ -D-galactopyranoside (without enzyme) is filled [26]. The respective flow rates orient on the protocol given above. 

Figure 4.43 Benchmarking of untreated and pre-treated Y-piece micro reactors to a commercial micro test-tube for the hydrolysis of p-nitrophenyl-/ -D-galactopyranoside. Y-piece micro reactor ( ) commercial micro test-tube ( ) pre-treated Y-piece microreactor (A) [26],...

W. Hakamata, T. Nishio, and T. Oku, Hydrolytic activity of a-galactosidases against deoxy derivatives of p-nitrophenyl a-D-galactopyranoside, Carbohydr. Res., 324 (2000) 107-115. 

Given the different chemical shifts of individual substrates and products, we believe there will be opportunities to use multiple reporters simultaneously. Indeed, we have investigated using 4-fluoro-2-nitrophenyl jS-o-galactopyranoside (PFONPG) and 2-fluorine-4-nitrophenyl S-o-galactopyranoside (OFPNPG) as... 

S. Yoon, H.G. Kim, K.H. Chun, J.E.N. Shin, 4-deoxy-analogs of p-nitrophenyl /i-D-galactopyranosides for specificity study with /J-galactosidase from escherichia coli. Bull. Korean Chem. Soc. 17 (1996) 599-604. 

W. Cui, P. Otten, Y. Li, K. Koeneman, J. Yu, R.P. Mason, A novel NMR approach to assessing gene transfection 4-fluoro-2-nitrophenyl-/ -D-galactopyranoside as a prototype reporter molecule for j -galactosidase, Magn. Reson. Med. 51 (2004) 616-620. 

L. Liu, V.D. Kodibagkar, J.-X. Yu, R.P. Mason, F-NMR detection of lacZ gene expression via the enzymic hydrolysis of 2-fluoro-4-nitrophenyl -o-galactopyranoside in vivo in PC3 prostate tumor xenografts in the mouse, Faseb J. 21(2007) 2014-2019. 

Following the work of Mclnnes,289,290 methanolysis in the presence of potassium carbonate was used to prepare methyl 6-O-acetyl- and methyl 6-0-octadecanoyl-/3-D-glucopyranoside,291 o-nitrophenyl /3-D-galactopyranoside 6-phosphate,292 methyl 5-thio-a-D-glucopyranoside 6-phosphate,293 and 6,6 -di-0-hexadecanoyl-a,a-trehalose,294 from the appropriate, per-0-(trialkylsilyl)ated precursors. 

Yeast /3-D-galactosidase hydrolyzes lactose and o-nitrophenyl /3-D-galactopyranoside (see Table XI), and also fluroan-3,6-diyl di(/3-D-ga-... 

AA AAm Con A DSS DSS-gel LCST MAPTAC MBA MP MP-gel NIPA ONPG PVMA SSPG Tc TMED acrylic acid acrylamide concanavalin A dextran sulfate sodium gel containing Con A/DSS complex lower critical solution temperature [(methacrylamide)propyl]trimethylammonium chloride /V,/V -methylenebis(acrylamide) a-methyl-D-mannopyranoside gel containing Con A/MP complex /V-isopropylacrylamide O-nitrophenyl-P-D-galactopyranoside poly(vinyl methyl ether) stimulus-sensitive polymer gel transition temperature /V,/V,/V, /V -tetramethylethylenediamine... 

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