GAG binding sites

GAG binding sites are generally identified by alanine scanning mutagenesis studies of the chemokine of interest. However, biochemical methods can be used to predict the regions that are involved, which can be subsequently confirmed by restricted mutagenesis. An example of such a method involves the use of protein bound to heparin beads, which is subsequently covalently stabilized by chemical... 

M-T7 Poxvirus Broad specificity CC, CXC, C chemokines Block GAG-binding site... 

A number of chemokines contain consensus sites for A-linked and/or 0-linked glycosylation. The naturally produced chemokines are heavily glycosylated but the bacterially produced recombinant proteins, which lack glycosylation, retain receptor-binding activity and induce calcium flux and chemotaxis both in vitro and in vivo. The in vivo function of glycosylation remains to be determined, but it may play a role in prolonging the chemokine half-life, and may improve GAG binding. 

Prevents early agent replication in the LRS, competes with GAG (glycosaminoglycan) binding site... 

Gag knuckle this fold is composed of short ( 20 amino acids) Cys2His2 zinc fingers from retroviral gag proteins Motif a primary, secondary, or tertiary structural domain Palindromic DNA-binding site a DNA sequence that contains one inverted repeat composed of two half-sites of nucleotides... 

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