Fume hood use

All operations should be done in a well-ventilated fume hood. Use care not to inhale vapors or get reactive silanes on your skin or in your eyes. 

Cyanogen bromide is an extremely toxic chemical and should be used only in a well-ventilated fume hood using the appropriate personal protective gear. The following protocol is based on the method of March et al. (1974), as recommended by Bang s Laboratories. 

Assemble a boiling water bath in a fume hood using a hot plate and a 250-mL beaker filled halfway with distilled water. 

CAUTION Hydrofluoric acid (HF) is highly toxic and corrosive, and severely irritating to the respiratory system and eyes All operations should be performed in a well-ventilated fume hood using appropriate safety precautions and procedures. 

Note Due to the high toxic nature of potassium osmate, it should be handled with extreme care in a chemical fume hood using chemical resistant gloves and safety goggles. 

Caution. The toxicity of the product is unknown. It should be handled in a fume hood using gloves. Methyllithium is extremely pyrophoric and moisture sensitive. 

The speed of homogenization should start slowly and then be gradually increased. This work should be performed in the fume hood using proper personal protection equipment (PPE). 

Extra Tip For an extra-tough stain, use a mixture of 3 mL of storage bottle strength (49%-52%) hydrofluoric acid to 100 mL of concentrated nitric acid. This solution should be used for only a minute or two at most and must be done in a fume hood. Use the same safety considerations that you would use for standard hydrofluoric acid.7... 

Carbon tetrachloride and tetramethylsilane (TMS) are both toxic chemicals see p. 197 for details about CCI4. TMS is volatile and must be kept in a tightly sealed container. Carry out all solution preparations in a fume hood. Use a pipette bulb do not pipette by mouth. Dispose of waste chemicals as instructed. 

Cadmium compounds are classified as potential carcinogens and should not be ingested or inhaled. All operations with Se and CdO must be done in a fume hood using gloves. 

PERSONAL PROTECTION use only in a chemical fume hood use self-contained breathing apparatus wear safety goggles, chemical resistant gloves and protective clothing. 

Caution. Carbonyl fluoride is a poisonous gas. Carbon monojode, which is highly poisonous, is a product of the reaction. Phosphorus-containing compounds are evil-smelling and harmful. These reactions should be carried out in a well-ventilated fume hood using safety shields. Skin should be protected from contact with reactants or products. 

Stainless steel Stainless steel may be attacked by some chemicals but type 316 stainless or equivalent is commonly used for the lining of perchloric acid hoods. Type 304 stainless may be used for radioisotope hoods which need to be easily decontaminated. Because of its vulnerability to some chemicals and its relatively high cost, it is not recommended for general purpose fume hood use. Among the problem chemicals for stainless steel are acids and compounds containing haUdes. 

Fume hoods used for radioactive matedals should be marked RADIOISOTOPE HOOD and in addition should be labeled with a CAUTION—RADIOACTIVE MATERIALS sign bearing the standard radiation symbol. The isotopes being used should be identified on the label. Under some circumstances, specialized additional signs may be needed. 

DAB = 3,3 -diaminobenzidine tetrahydrochloride dihydrate. Cat. no. 5972SA from BRL. Caution Handle with extreme care in fume-hood using protection for eyes and hands. For a stock solution, add 10 mL of 0.05MTris-HCl buffer, pH 7.6, to 100 mg DAB powder. Mix well. Transfer in 1-mL aliquots to Eppendorf tubes. Wrap each Eppendorf tube in foil (DAB is light sensitive) and store at -20 C. A 0.05% solution should be made up just before use Mix 575 pL of 0.05M Tris-HCl, pH 7.6,0.OIM imidazole, plus 33.75 pL DAB stock solution and 18.75 pL hydrogen peroxide. 

Each chemical fume hood tested within last year Sash closed when not in active use Chemical-fume hood vents (baffles) unobstructed Chemical-fume hood used with sash in appropriate position Chemical storage limited in actively used hood Chemicals and equipment at least 6 in. firom the sash... 

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