Full tandem mass spectrometric

Figure 3.32 Full-mass spectra at peak maxima of the constant-neutral-loss TIC trace shown in Figure 3.31(a), obtained after (a) 13.25 and (b) 15.9 min, from the LC-MS analysis of a mixture of atrazine and its degradation products. Reprinted from J. Chro-matogr., A, 915, Steen, R. J. C. A., Bobeldijk, I. and Brinkman, U. A. Th., Screening for transformation products of pesticides using tandem mass spectrometric scan modes , 129-137, Copyright (2001), with permission from Elsevier Science.

Traditional methodologies for structural identification of trace level impurities in drng substances/products usually involve fractionation of each impurities using a scaled-np analytical chromatographic method, followed by off-line spectroscopic analysis. Coupling of HPLC separation and electrospray mass spectrometry allows on-line acquisition of full scan mass spectra and generation of tandem mass spectrometric data. LC/ESI MS has revolntionized trace analysis for qnalitative and quantitative studies in pharmaceutical analysis. 

Mullen W, Boitier A, Stewart AJ, Crozier A. 2004. Flavonoid metabolites in human plasma and urine after the consumption of red onions Analysis by liquid chromatography with photodiode array and full scan tandem mass spectrometric detection. J Chromatogr A 1058 163-168. 

Ito, H., Yamaguchi, H., Fujikawa, A., Tanaka, N., Furugen, A., Miyamori, K., Takahashi, N., Ogura, J., Kobayashi, M., Yamada, T., Mano, N., Iseki, K. A full validated hydrophilic interaction hquid chromatography-tandem mass spectrometric method for the quantification of oxaliplatin in human plasma ultrafiltrates. J. Pharm. Biomed. Anal. 71, 99-103 (2012)... 

Eor direct characterization of disubstituted piperidine alkaloids in extracts of L. inflata, tandem mass spectrometric method was developed using electrospray ionization. Analysis was performed in positive ion mode on a triple quadropole LC/MS system. The identification and structural elucidation of the alkaloids were performed by comparing their changes in molecular mass (AM), full-scan MS-MS spectra with those of lobeline, lobelanine, norlobelanine, and lobelanidine. These alkaloids and ten other derivatives were identified in the plant extracts. 

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