Triple quadropole

Cole, M. Enke, C. Direct determination of phospholipid structures in microorganisms by fast atom bombardment triple quadropole mass spectrometry. Anal. Chem. 1991, 63,1032-1038. 

HPLC column was directed into a triple quadropole mass spectrometer equipped with an atmospheric pressure articulated ion spray (electrospray) source. Using MS-MS conditions, dissociation of the parent ions yielded daughter ions comparable to the IM+H]+ of PAF and other molecules, such as O-phosphocholine. Quantitative analyses were obtained by selective ion monitoring. Amounts as low as 0.3 ng of PAF and similar compounds were detected. Interestingly with the lyso derivatives, the limited of detection was 3 ng. The primary alkyl chain lengths present in the neutrophil-derived PAF were 16 0 and 18 0. No mention was made of the presence of any 1-0-long chain acyl analogs. 

Separate the formed ions according to their mass-to-charge ratio (m/z ratio). This takes place in the mass analyzer. By far, the most used analyzer is the quadropole, either as single or as triple quadropole, combined with a soft ioniza-... 

Fig. 1 Schematic representation of a mass spectrometer depicting its main components and the different modes used. Abbreviations DIP direct insertion probe DEP direct exposure probe GC gas chromatography LC liquid chromatography CE capillary chromatography TEC thin-layer chromatography FEE field-flow fractionation APCI atmospheric pressure ionization El electron impact Cl chemical ionization FAB fast-atom bombardment PD plasma desorption MALDI matrix-assisted laser desorption ionization ED laser desorption TSP thermospray ESI electron spray ionization HSI hypherthermal surface ionization Q quadropole QQQ triple quadropole TOE time-of-fiight FTMS Fourier transform mass spectrometer IT ion trap EM electrom multiplier PM photomultiplier ICR ion cyclotron resonance.

FIGURE 9.15 Schematic diagram of aspiration-type IMS interface to a quadrupole mass spectrometer. (From Adamov et al., Interfacing an aspiration ion mobility spectrometer to triple quadropole mass spectrometer, Rev. Sci. Instrum. 2007,78(4) 044101. With permission.)... 

Eor direct characterization of disubstituted piperidine alkaloids in extracts of L. inflata, tandem mass spectrometric method was developed using electrospray ionization. Analysis was performed in positive ion mode on a triple quadropole LC/MS system. The identification and structural elucidation of the alkaloids were performed by comparing their changes in molecular mass (AM), full-scan MS-MS spectra with those of lobeline, lobelanine, norlobelanine, and lobelanidine. These alkaloids and ten other derivatives were identified in the plant extracts. 

Gobey, J. Cole, M. Jamszewski, J. Covey, T. Chau, T. Kovarik, P Corr, J. Characterization and performance of MALDI on a triple quadropole mass spectrometer for analysis and quantification of small molecules. Anal. Chem. 2005, 77(17), 5643-5654. 

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