Full Genome Sequencing

Since this technique was pioneered the speed of automated DNA sequence determination has increased dramatically to the point that the same sequence is routinely determined many times so that a consensus sequence results with as few errors as possible. 

The key to making such approaches useful is to link the enhanced concentration of a specific sequence with an output that can be measured. More importantly the technology used to detect and report the output signal must be relatively cheap and easy to use if it is to be widely adopted. The cost of a personal genome determination is now relatively low, to the extent individuals underlying medical conditions have been diagnosed based on their symptoms and rapid sequencing. 

Researchers are actively pursuing a method of reading the sequence of bases on DNA in a rapid manner. Some advances have already been made in this area due to 

---

Various signaling systems used by animal cells also have analogs in the prokaryotes. As the full genomic sequences of more, and more diverse, bacteria become known, researchers have discovered genes that encode proteins similar to protein Ser or Thr kinases, Ras-like proteins regulated by GTP binding, and proteins with SH3 domains. Receptor Tyr kinases have not been detected in bacteria, but (P)-Tyr residues do occur in some bacterial proteins, so there must be an enzyme that phosphorylates Tyr residues. 

A review of platyhelminth genomes written just 10 years ago would have painted a very different picture to the one which we can write today and we would never then have predicted that we would be in the position that we currently find ourselves in, with full genome sequencing projects underway for several flat-worm species. Even if one s scientific interest falls outside the currently targeted species, these efforts should not just be viewed as stamp collecting they are revealing fundamental information of potential interest and value to the entire flatworm research community. Moreover, the methods of analysis are... 

Fig. 8.7 The principle of full genome sequencing using labelled dideoxy (dd) nucleotides labelled fragments are eluted through a gel and detected in order of length to determine the complementary DNA sequence from which the original can be deduced...

The default region to be displayed is Full sequence. Because we wish to view a comparison between the full genome sequences, leave this option selected, and click the Next button. 

---