FTIR micro

Boydston-White, S., Romeo, M.) Chernenko, T Regina, A., Miljkovic, M. and Diem, M. (2006) Cell-cycle-dependent variations in FTIR micro-spedra of single proliferating HeLa cells principal component and artificial neural network analysis. Biochim. Biophys. Acta, 1758 (7), 908-14. 

Isomorphous substitution of boron into the MFI structure to produce [B]ZSM-5 was carried out and investigated via FTIR micro-spectroscopy ( infrared microscopy ) of single crystals by Jansen et al. [308]. Typical bands were observed at 1380 and 905 cm. The integrated band intensity of the 905 cm" band was used as a quantitative measure of the boron content, and the boron distribution in the single crystals was shown to be homogeneous. 

Model composites of Kevlar-49 and D-glass fibre/ epoxy systems were analysed using FTIR micro-... 

Pentoney et al used a similar capillary SFC-FTIR micro-spectrometric... 

PA-11 or PA-66 or PA-6,10 or PA-6,12/block copolyetherester Morphology/mechanical properties/DSC/ rheology/selective solvent extraction/ FTIR/micro-Raman spectroscopy Koulouri et al. 1999b... 

A further advantage of supercritical carbon dioxide is its volatility on decompression. Fourier transform infrared (FTIR) micro spectrometry can therefore be coupled to SFC with ease as there is no significant interference from the mobile phase [17, 18]. With this technique, each eluate is directly deposited from the end of a restrictor onto a small area of an infrared (IR)-transparent support as the mobile phase evaporates away. The support is then positioned in an IR microscope, which serves as a beam condenser, matching the IR beam size to the area that the sample occupies (typically 200 pm in diameter). In this way IR spectra can be collected from nanogram levels of each deposited eluate. The information yielded by combining the separation power of CSFC with the identification power of FTIR micro spectrometry greatly increases the possibility of elucidating the structures of unknown compounds. 

However, over the last decade, important advances have been made in IR micro-spectroscopy with a synchrotron source [16], which can provide new opportunities and motivation for the study of polymeric materials. The most utilized key synchrotron parameter for this community is the ability, thanks to the source brightness, to differentiate the chemical nature of the constituents in multicomponent polymeric systems. When the sizes of the different domains are in the range of the IR wavelength (micron scale), the spatial differentiation and study of each of these domains is possible using SR-FTIR micro-spectroscopy. Such analytical tools allow the study of various aspects related to the chemical composition, structure and morphology of the polymeric materials. Some of the areas that have benefited from synchrotron infrared over the last four years are reviewed in this section. 

Fig. 3 illustrates the analysis of such a multi-layered glitter particle using SR-FTIR micro-spectroscopy [24]. 

Compared with conventional FTIR, micro-FTIR can offer additional spatial resolution (Endoh et al. 2007). The composition change from the anode to the cathode side can be monitored on membrane cross sections. By monitoring of the carboxylic add concentration (l,690cm ), it was found that anode degradation is more severe than cathode degradation (Fig. 12). 

Three crystalline modifications of fluocinolone acetonide (Forms A, B and C) were characterised by FTIR, DSC, TG - FTIR, micro-FTIR and XRD [21]. On heating Form A and Form C were converted into Form B. Polymorph A was enantiotropically related to Form B while Form C was monotropically related to B[21]. Giron et al [22] examined tetracaine hydrochloride. Ten different forms were identified six anhydrous crystalline forms, an amorphous form, a hemihydrate, a monohydrate and a tetrahydrate. The anhydrous Form I is the stable thermodynamic modification at ambient temperature. Form 2 was reversibly enantiotropic to Form I. The other four modifications (3, 4, 5 and 6) were monotropic polymorphs of Form I. Only Form 1 and Form 5 were stable at room temperature [22]. 

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