Fragments immunoglobulin

Paramagnetic ion probes have been successfully used to study the binding characteristics and solution conformations of a number of biochemically important molecules. These include vitamin D, (533) penicillins, (534) and the antibiotics tetracycline, (535-537) vancomycin, (632) and bacitracin. (633) Antibodies and antibody fragments (immunoglobulins, IgG) have been studied by proton relaxation enhancement methods when lanthanide ions, particularly Gd(m), are bound to the proteins. (746-748). 

Pick RB Jr, Naegel GP, Squier SU, Wood RE, Gee JB, Reynolds HY. Proteins of the cystic fibrosis respiratory tract. Fragmented immunoglobulin G opsonic antibody causing defective opsonophagocytosis. J Clin Invest 1984 74 236. 

Figure 1S.6 Enzymatic cleavage of immunoglobulin IgG. The enzyme papain splits the molecule in the hinge region, yielding two Fab fragments and one Fc fragment.

Wang, J., et al. Atomic structure of a fragment of human CD4 containing two immunoglobulin-like domains. Nature 348 411-418, 1990. 

CN immunoglobulin G (human-mouse monoclonal c7E3 clone p7E3VHhCy4 Fab fragment antihuman... 

As depicted in Figure 50-8, digestion of an immunoglobulin by the enzyme papain produces two antigen-binding fragments (Fab) and one crystallizable fragment (Fc), which is responsible for functions of im-... 

The constant regions of the immunoglobulin molecules, particularly the C 2 and 0 3 (and C 4 of IgM and IgE), which constitute the Fc fragment, are responsible for the class-specific effector functions of the different immunoglobulin molecules (Table 50-7, bottom part), eg, complement fixation or transplacental passage. 

IgG consists of four polypeptide subunits held together by disulphide bonds. Native immunoglobulins are rather resistant to proteolytic digestion but certain enzymes have been usefiil in elucidating their structure. Papain cleaves the molecule into three fragments of similar size ... 

FAB Fast-electron bombardment Fab Antigen binding fragment F(ab )2 Fragment of an immunoglobulin produced pepsin treatment... 

Wochner, R.D. Strober, W. and Waldman, T.A. The role of the kidney in catabolism of Bence Jones proteins and immunoglobulin fragments. J. Fxper Med 126 207-221, 1967. 

Edelman, G.M., Gall, W.E., Waxdal, M.J., and Konigsberg, W.H. (1968) The covalent structure of a human gG-immunoglobulin. I. Isolation and characterization of the whole molecules, the polypeptide chains, and the tryptic fragments. Biochemistry 7, 1950-1958. 

Martin, F.J., and Papahadjopoulos, D. (1982) Irreversible coupling of immunoglobulin fragments to preformed vesicles./. Biol. Chem. 257, 286-288. 

L. Korecka, Z. Bilkova, M. Holeapek, J. Kralovsky, M. Benes, J. Lenfeld, N. Mine, R. Cecal, J.-L. Viovy, and M. Przybylski, Utilization of newly developed immobilized enzyme reactors for preparation and study of immunoglobulin G fragments. Journal of Chromatography, B Analytical Technologies in the Biomedical and Life Sciences 808, 15-24 (2004). 

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